Mouse anti-Human CD11a (LFA-1 alpha) Monoclonal Antibody

Mouse Anti-Human CD11a/LFA-1a-LE/AF

Cat. Num. AAA674107

• Reactivity: Human
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD11a (LFA-1 alpha); Monoclonal Antibody; Mouse Anti-Human CD11a/LFA-1a-LE/AF; Mouse Anti-Human CD11a/LFA-1a - Low Endotoxin/Azide-Free; Mouse Anti-Human CD11a (LFA-1 alpha); anti-CD11a (LFA-1 alpha) antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 38
• Specificity: Human CD11a (LFA-1 alpha); CD11a, also known as leukocyte function-associated antigen-1 (LFA-1 alpha), represents the 180 kDa integrin alpha L subunit which combines with the CD18 beta 2 integrin subunit to form the alpha L beta 2 integrin heterodimer. It is expressed on lymphocytes, granulocytes, monocytes, and macrophages and is upregulated on activated T cells. CD11a/LFA-1 alpha mediates adhesion of lymphocytes to vascular endothelium and is involved in costimulation.1-5
• Form/Format: Low Endotoxin, Azide - Free (LE/AF)

• Applicable Applications for anti-CD11a (LFA-1 alpha) antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Application Notes: Research Application(s): Studies in cell adhesion and cytotoxicity; Immunoprecipitation; Blocking studies
• Characterization: To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
• Preparation and Storage: The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin/azide-free antibody is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of PBS; aliquot and freeze upon receipt. ; Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

References

1. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD11a Section, Academic Press, New York, p. 156.
2. Dransfield, I., and N. Hogg. 1989. EMBO J. 12:3759.
3. Springer, T.A. 1990. Nature 346:425.
4. Springer, T.A. 1994. Cell 76:301.
5. Lub, M., Y. van Kooyk, and C.G. Figdor. 1995. Immunol. Today 16:479.