Mouse anti-Human CD11a / Integrin alphaL / LFA-1 alpha Chain Monoclonal Antibody | anti-ITGAL antibody

CD11a / Integrin alphaL / LFA-1 alpha Chain Mouse Monoclonal Antibody

Cat. Num. AAA439260

• Gene Names: ITGAL; CD11A; LFA-1; LFA1A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD11a / Integrin alphaL / LFA-1 alpha Chain; Monoclonal Antibody; CD11a / Integrin alphaL / LFA-1 alpha Chain Mouse Monoclonal Antibody; Antigen CD11A (p180); lymphocyte function associated antigen 1; alpha polypeptide; Integrin alpha-L; ITGAL; Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain; LFA 1 alpha (LFA1A); Ly15; Ly21; p180; anti-ITGAL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: DF1524
• Specificity: Recognizes a protein of 180kDa, identified as CD11a (Leucocyte Workshop IV; Code 1524). CD11a complex with the 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses. This MAb potently blocks LFA-1 dependent homotypic cell aggregation.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1086

• Applicable Applications for anti-ITGAL antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Functional Studies (Order Ab without BSA & Azide)
• Cellular Localization: Cell Surface
• Immunogen: Human CD11a protein
• Hu-Chromosome Location: 16p11.2
• Positive Control: JY, HUT-78, MOLT-4, HL60, U937, and human leukocytes. Human lymph nodes and tonsils.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Knapp et al. (Eds.), Leukocyte Typing IV, 1989: Sections N1 (pp. 543.551), N1.l (pp. 551553); N1.2 (pp. 553554); N1.3 (pp. 555558); N1.8 (pp. 566570); N1.9 (pp. 570574); N14.4 (pp. 689693). Petruzzelli L et al. Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18. J Immunol 1995, 155(2):854-866. Edwards CP et al. Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1. J Biol Chem 1998, 273(44):28937-44. Shang XZ and Issekutz AC. Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol 1998, 28(6):1970-1979. Tian L et al. The neuronal glycoprotein telencephalin is a cellular ligand for the CD11a/CD18 leukocyte integrin. J Immunol 1997, 158(2):928-936

NCBI and Uniprot Product Information

• NCBI GI #: 167466217
• NCBI GeneID: 3683
• NCBI Accession #: NP_001107852.1
• NCBI GenBank Nucleotide #: NM_001114380.1
• UniProt Accession #: P20701; O43746; Q45H73; Q96HB1; Q9UBC8
• Molecular Weight: 180kDa
• NCBI Official Full Name: integrin alpha-L isoform b
• NCBI Official Synonym Full Names: integrin subunit alpha L
• NCBI Official Symbol: ITGAL
• NCBI Official Synonym Symbols: CD11A; LFA-1; LFA1A
• NCBI Protein Information: integrin alpha-L
• UniProt Protein Name: Integrin alpha-L
• Protein Family: Integrin
• UniProt Gene Name: ITGAL
• UniProt Synonym Gene Names: CD11A; LFA-1A
• UniProt Entry Name: ITAL_HUMAN

NCBI Description

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGAL: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Belongs to the integrin alpha chain family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell surface; Cell adhesion

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; membrane; plasma membrane

Molecular Function: cell adhesion molecule binding; ICAM-3 receptor activity; protein binding

Biological Process: cell motility; cell-matrix adhesion; extracellular matrix organization and biogenesis; heterophilic cell adhesion; leukocyte adhesion; leukocyte migration; receptor clustering; regulation of immune response; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell