Mouse CD105 Monoclonal Antibody | anti-CD105 antibody

MOUSE ANTI HUMAN CD105

Cat. Num. AAA212208

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Cynomolgus monkey, Horse, Monkey, Primate, Rhesus Monkey
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: CD105; Monoclonal Antibody; MOUSE ANTI HUMAN CD105; anti-CD105 antibody

• Host: Mouse
• Reactivity: Cynomolgus monkey, Horse, Monkey, Primate, Rhesus Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SN6
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 625

• Applicable Applications for anti-CD105 antibody: Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.; Flow Cytometry: Maximum Dilution: 1/50
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: Partially purified cell membrane antigens from fresh leukaemia cells.; Fusion Partners
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488 (MBS212208A488))

Testing Data

(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Expression of endothelial antigens in endothelium-adherent monocytes in co-culture with HCAECs.HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HCAECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by dual-colour flow cytometry for HLA-A2 and (A) CD105, (B) eNOS and (C) VEGFR2 expression on Day 2 of co-culture.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Testing Data

(Staining of KG1 cells with Mouse anti Human CD105)

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647 (MBS212208A647))

Testing Data

(Staining of KG1 cells with Mouse anti Human CD105RPE (MBS213632))

Testing Data

(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Increased expression of CD105 and CD144 in the endothelium-adherent monocytes during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were maintained in co-culture up to Day 6, then assessed by dual-colour flow cytometry for HLA-A2 and (A) CD105 and (B) CD144 expression on Day 3 of co-culture. Representative plots from 4 -7 individual experiments are shown. The increase in CD105 from Day 0 to Day 6 (C) and CD144 expression from Day 0 to Day 6 (D) is also shown.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Testing Data

(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry on brain microvascular endothelial cellsImage caption:Characterization and functional features of human and murine BMVECs. (A) Phase contrast micrographs of confluent monolayers of human (left image) and murine (right image) BMVECs. BMVECs present the typical œcobblestone appearance. Scale bar, 100 um and 200 um for human BMVECs and murine BMVECs. B) Human (left image) and murine (right image) BMVECs showed a clear cytoplasmic staining for CD31. Scale bar, 50 um. C) Human (left image) and murine (right image) cells displayed an intense positive immunofluorescence for vWf. Scale bar, 50 um. D) Flow cytometric analysis of BMVECs. Human BMVECs resulted positive (gray histograms) for CD31 (left graph), CD105, CD146 (left gaph), UEA-1 staining; murine BMVECs resulted positive for CD31 (right graph), CD34, CD146 (right graph) and Tie-2 staining. White histograms represent the isotype controls of each antibody. E) Capillary tube-like structure produced by human (left image) and murine (right image) BMVECs, 7 h after plating onto Matrigel. Scale bar, 100 um. F) LDL-uptake assay on human (left image) and murine (right image) BMVECs. Scale bar, 50 um. G) Human (left image) and murine (right image) BMVECs were labelled for GLUT-1. Scale bar, 50 um. H) Immunofluorescence for eNOS in human (left image) and murine (right image) BMVECs. Scale bar, 50 um. All nuclei were counterstained with DAPI (blue). One representative of three independent experiments performed in blind is shown for each figure.From: Navone SE, Marfia G, Nava S, Invernici G, Cristini S, Balbi S, Sangiorgi S, Ciusani E, Bosutti A, Alessandri G, Slevin M, Parati EA. Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival. Vasc Cell. 2013 May 14;5(1):10.)

Testing Data

(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Endothelial adherence of blood monocytes. PBMCs were isolated from HLA-A2+ donors and incubated with HLA-A2- HUVECs (1x106 cells/well) for 2 h, after which the non-adherent PBMCs were removed by washing. The co-cultured cell layers were immediately analysed with dual-colour flow cytometry for HLA-A2 and (A) CD34, (B) CD14, (C) CD11b, (D) CD16, (E) CD105 and (F) CD144 expression. Representative plots from 4 -6 individual experiments are shown. (G) Two parameters dot plot showing typical isotype controls.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

Testing Data

(Staining of KG1 cells with Mouse anti Human CD105:FITC (MBS210726))

Testing Data

(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

NCBI and Uniprot Product Information

• NCBI GI #: 4557555
• NCBI GeneID: 2022
• NCBI Accession #: NP_000109.1
• NCBI GenBank Nucleotide #: NM_000118.3
• Molecular Weight: 67,542 Da
• NCBI Official Full Name: endoglin isoform 2
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin; CD105 antigen
• UniProt Protein Name: Endoglin
• UniProt Gene Name: ENG
• UniProt Synonym Gene Names: END
• UniProt Entry Name: EGLN_HUMAN

NCBI Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Uniprot Description

ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: nucleoplasm; extracellular space; focal adhesion; cell surface; cytoplasm; receptor complex; external side of plasma membrane

Molecular Function: transforming growth factor beta receptor activity; protein binding; transmembrane receptor activity; protein homodimerization activity; glycosaminoglycan binding; transforming growth factor beta binding; galactose binding; punt binding; activin binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: wound healing; positive regulation of collagen biosynthetic process; cell motility involved in cell locomotion; positive regulation of systemic arterial blood pressure; negative regulation of transcription from RNA polymerase II promoter; response to corticosteroid stimulus; BMP signaling pathway; smooth muscle development; extracellular matrix disassembly; regulation of transforming growth factor beta receptor signaling pathway; venous blood vessel morphogenesis; central nervous system vasculogenesis; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; heart looping; vasculogenesis; cell adhesion; chronological cell aging; negative regulation of cell migration; positive regulation of BMP signaling pathway; regulation of cell adhesion; cell migration; negative regulation of nitric-oxide synthase activity; regulation of phosphorylation; regulation of cell proliferation; patterning of blood vessels; negative regulation of endothelial cell proliferation; response to hypoxia; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway

Disease: Telangiectasia, Hereditary Hemorrhagic, Of Rendu, Osler, And Weber