Testing Data
Mouse anti-Human CD105 Monoclonal Antibody | anti-CD105 antibody
Monoclonal anti-human CD105 (Endoglin)/APC
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
CD105; Monoclonal Antibody; Monoclonal anti-human CD105 (Endoglin)/APC; anti-CD105; anti-human CD105; Endoglin; anti-CD105 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
SN6
Specificity
Antibody SN6 recognizes an extracellular epitope of the human CD105 homodimer with a molecular weight of about 160 kd. It has been reported to cross react with non human Primate as well as Equine and Porcine (11) CD105.
Form/Format
50mM Sodium Phosphate pH 7.5, 500mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2%BSA, 0.04% NaN3 (as a preservative).
Concentration
Volume in Vial: 0.2 ml (varies by lot)
Applicable Applications for anti-CD105 antibody
Flow Cytometry (FC/FACS) (12), Immunohistochemistry (IHC)
Application Notes
Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
Immunogen
Membrane preparation of human B-lineage leukemia cells
Conjugation
Allophycocyanin (APC)
Production
Protein A purified antibody from tissue culture supernatant was conjugated to cross-linked Allophycocyanin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5mg/ml with an A650/A280 ratioof 1.96.
Performance
Five x 105 cultured human Nalm-6cells per tube were washed and incubated 45 minutes on ice with 80ul of anti-CD105/APC at a dilution factor of 1:50 (10ug/ml). Cells were washed three times, fixed and analyzed byFACS. Cells stained positive with a mean shift of 1.55 log10 fluorescent units when compared to a Mouse IgG1/APC negative control (#MBS666633). Binding was blocked when cells were preincubated 10 minutes with 20ul of 0.5mg/ml anti-CD105 antibody (#MBS666489).
Preparation and Storage
Store at 2-5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD105 antibody
Human CD105 (endoglin) is a homodimeric membrane glycoprotein (1) virtually restricted to endothelial cells, and present on some leukemia cells and minor subsets of bone marrow cells. CD105 binds to TGF-b1 and b3 with high affinity (4). Antibody SN6 will augment binding of TGF-b1 to CD105 expressing leukemia cells. Antibody SN6 has been used to identify mesenchymal progenitor cells populations (8,9,10)
References
1. Y. Haruta & B.K. Seon, (1986) Proc Natl Acad Sci USA 83: 7898-7902.
2. H. Hara, et al, (1988) Cancer Res 48: 4673-4680.
3. W.C. Biddle, et al, (1989) Leukemia Res 13: 699-707.
4. S. Cheifetz, et al, (1992) J Biol Chem 267: 19027-19030.
5. B.K. Seon, et al, (1995) Proc Am Assoc Can Res 36: 23.
2. H. Hara, et al, (1988) Cancer Res 48: 4673-4680.
3. W.C. Biddle, et al, (1989) Leukemia Res 13: 699-707.
4. S. Cheifetz, et al, (1992) J Biol Chem 267: 19027-19030.
5. B.K. Seon, et al, (1995) Proc Am Assoc Can Res 36: 23.
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Product Notes
The CD105 (Catalog #AAA666951) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal anti-human CD105 (Endoglin)/APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD105 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (12), Immunohistochemistry (IHC). Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD105 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD105, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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