Mouse CD100 Monoclonal Antibody | anti-SEMA4D antibody

CD100 (Semaphorin-4D) Mouse Monoclonal Antibody

Cat. Num. AAA439741

• Gene Names: SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• Reactivity: Human, Baboon.; Does not react with Horse.; Others not tested
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD100; Monoclonal Antibody; CD100 (Semaphorin-4D) Mouse Monoclonal Antibody; COLL4; Collapsin4; GR3; M sema G; MSEMA; SEMA4D; Semacl2; SemaH; SEMAJ; Semaphorin C like 2; Semaphorin H; Semaphorin J; Semaphorin-4D; Semcl2; anti-SEMA4D antibody

• Host: Mouse
• Reactivity: Human, Baboon.; Does not react with Horse.; Others not tested
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: 133-1C6
• Specificity: Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100 (Workshop VI; Code N-L026). It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 738

• Applicable Applications for anti-SEMA4D antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)
• Cellular Localization: Cell Surface
• Immunogen: PHA stimulated human peripheral blood lymphocytes.
• Hu-Chromosome Location: 9q22.2
• Positive Control: Daudi, Raji, HUT-78, Kg1a, U937, and human lymphocytes. Human tonsils and lymph nodes.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Kishimoto T et al. (eds) Leukocyte Typing VI, Garland Publishing, New York, 1997. Schlossman SF et al. (eds) Leucocyte Typing V Oxford University Press, Oxford. 1995. Delaire S et al. CD100 is a leukocyte semaphorin. Cell Mol Life Sci 1998, 54(11):1265-1276. Dorfman DM et al. The leukocyte semaphorin CD100 is expressed in most T-cell, but few B-cell, non-Hodgkin's lymphomas. Am J Pathol 1998, 153(1):255-262. Herold C et al. CD100 is associated with CD45 at the surface of human T lymphocytes. Role in T cell homotypic adhesion. J Immunol 1996, 157(12):5262-5268. Elhabazi A et al. The human semaphorin-like leukocyte cell surface molecule CD100 associates with a serine kinase activity. J Biol Chem 1997, 272(38):23515-23520. Hall KT et al. Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. Proc Natl Acad Sci USA 1996, 93(21):11780-1178

NCBI and Uniprot Product Information

• NCBI GI #: 214010220
• NCBI GeneID: 10507
• NCBI Accession #: NP_001135759.1
• NCBI GenBank Nucleotide #: NM_001142287.1
• UniProt Accession #: Q92854; Q7Z5S4; Q8N8B0; B2RPM6
• Molecular Weight: 50kDa (Monomer)
• NCBI Official Full Name: semaphorin-4D isoform 2
• NCBI Official Synonym Full Names: semaphorin 4D
• NCBI Official Symbol: SEMA4D
• NCBI Official Synonym Symbols: CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• NCBI Protein Information: semaphorin-4D
• UniProt Protein Name: Semaphorin-4D
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA4D
• UniProt Synonym Gene Names: C9orf164; CD100; SEMAJ
• UniProt Entry Name: SEM4D_HUMAN

Uniprot Description

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: chemorepellent activity; protein binding; receptor activity; receptor binding; semaphorin receptor binding; transmembrane receptor activity

Biological Process: cell adhesion; immune response; negative chemotaxis; negative regulation of apoptosis; negative regulation of axon extension involved in axon guidance; negative regulation of cell adhesion; negative regulation of osteoblast differentiation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; neural crest cell migration; positive regulation of cell migration; positive regulation of collateral sprouting; positive regulation of GTPase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; regulation of cell projection organization and biogenesis; regulation of cell shape; regulation of dendrite morphogenesis