Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control (biotin) (open histogram) or 0.25 ug of CD10 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Cells in the large scatter population were used for analysis.)
Mouse CD10 Monoclonal Antibody | anti-CD10 antibody
CD10 antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD10; Monoclonal Antibody; CD10 antibody (biotin); Monoclonal CD10; Anti-CD10; Neprilysin; Mme; Common Acute Lymphocytic Leukemia antigen; CALLA; CD-10; CD 10; NEP; anti-CD10 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
CB-CALLA
Specificity
Human
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
5 ul (1.0 ug)/test (varies by lot)
Applicable Applications for anti-CD10 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: 5 ul (1.0 ug)/test
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Biological Significance
The CB-CALLA monoclonal antibody recognizes human CD10, which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias. Subsequently, CD10 was shown to be the same molecule as the neutral endopeptidase, or KII-NA. CD10 is a Zn2+-dependent metallo-peptidase with endothelin, glucagon, gastrin, neurotensin and bradykinin included among its substrates. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and expression has also been reported on mature B cells in germinal centres. In addition to the hematopoietic compartment, other major sites of CD10 expression are the brush border of enterocytes and renal tubules and glomeruli. There is partial blocking of the CB-CALLA and MEM-78 monoclonal antibodies indicating that they recognize similar epitopes.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control (biotin) (open histogram) or 0.25 ug of CD10 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Cells in the large scatter population were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control (biotin) (open histogram) or 0.25 ug of CD10 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Cells in the large scatter population were used for analysis.)
Related Product Information for anti-CD10 antibody
Mouse monoclonal CD10 antibody (biotin)
Product Categories/Family for anti-CD10 antibody
Similar Products
Product Notes
The CD10 (Catalog #AAA834966) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD10 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: 5 ul (1.0 ug)/test A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Researchers should empirically determine the suitability of the CD10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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