Mouse CCAR2 Monoclonal Antibody | anti-CCAR2 antibody

Mouse Anti CCAR2

Cat. Num. AAA225708

• Gene Names: CCAR2; DBC1; DBC-1; NET35; p30DBC; p30 DBC; KIAA1967
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified IgG prepared by affinity chromatography
• Synonyms: CCAR2; Monoclonal Antibody; Mouse Anti CCAR2; CCAR2 antibody (3G4-D11-D7); anti-CCAR2 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3G4-D11-D7
• Purity/Purification: Purified IgG prepared by affinity chromatography
• Form/Format: Liquid; TRIS Buffered Glycine
• Sequence Length: 923

• Applicable Applications for anti-CCAR2 antibody: Western Blot (WB)
• Immunogen: Recombinant human CCAR2 protein fragments expressed in E Coli.
• Preservative Stabilizers: 0.2% Sodium Azide (NaN3); 50% Glycerol
• Preparation and Storage: Store at -20 degree C only.; Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.; Should this product contain a precipitate we recommend microcentrifugation before use.

Western Blot (WB)

(Western blot analysis of various cell lysates using Mouse anti CCAR2 antibody)

Related Product Information for anti-CCAR2 antibody

Mouse anti CCAR2 antibody recognizes cell cycle and apoptosis regulator protein 2 (CCAR2), also known as DBC1 and KIAA1967. The Dbc1 gene was discovered to be homozygously deleted in human chromosome 8p21 in breast cancer (Hamaguchi et al. 2002). CCAR2 regulates ER beta function (Koyama et al. 2010), heterochromatin formation (Li et al. 2009) and negatively regulates the NAD-dependent protein deacetylase sirtuin-1 (SIRT1) (Kim et al. 2008). In addition, DBC1 has also been shown to act as a tumor suppressor by regulating p53 binding; CCAR2 competes with MDM2 for binding to p53 (Qin et al. 2015).

NCBI and Uniprot Product Information

• NCBI GI #: 24432106
• NCBI GeneID: 57805
• NCBI Accession #: NP_066997.3
• NCBI GenBank Nucleotide #: NM_021174.5
• UniProt Accession #: Q8N163
• NCBI Official Full Name: cell cycle and apoptosis regulator protein 2 isoform 1
• NCBI Official Synonym Full Names: cell cycle and apoptosis regulator 2
• NCBI Official Symbol: CCAR2
• NCBI Official Synonym Symbols: DBC1; DBC-1; NET35; p30DBC; p30 DBC; KIAA1967
• NCBI Protein Information: cell cycle and apoptosis regulator protein 2
• UniProt Protein Name: DBIRD complex subunit KIAA1967
• Protein Family: Cell cycle and apoptosis regulator protein
• UniProt Gene Name: KIAA1967
• UniProt Synonym Gene Names: DBC1; DBC-1; DBC.1
• UniProt Entry Name: K1967_HUMAN

Uniprot Description

DBC-1: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. Component of the DBIRD complex. Interacts with ZNF326/ZIRD; the interaction is direct. Interacts (via N-terminus) with SIRT1. Interacts (via N-terminus) with SUV39H1; this interaction abolishes the interaction with SIRT1. Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nuclear receptor co-regulator; Mitochondrial; Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: nucleoplasm; spliceosome; mitochondrial matrix; nuclear chromatin; cytoplasm; nucleus

Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding

Biological Process: positive regulation of apoptosis; RNA splicing; negative regulation of catalytic activity; rhythmic process; regulation of protein stability; mitochondrial fragmentation during apoptosis; mRNA processing; regulation of circadian rhythm; cell cycle; response to DNA damage stimulus; regulation of RNA elongation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; response to UV