Mouse CBP, p300 Monoclonal Antibody | anti-CREBBP antibody

CBP, p300 (CREB Binding Protein, Histone Acetyl Transferase, RTS, RSTS, Rubinstein-Taybi Syndrome) (BSA & Azide Free)

Cat. Num. AAA631812

• Gene Names: CREBBP; CBP; RSTS; KAT3A
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CBP; p300; Monoclonal Antibody; p300 (CREB Binding Protein; Histone Acetyl Transferase; RTS; RSTS; Rubinstein-Taybi Syndrome) (BSA & Azide Free); Anti -CBP; anti-CREBBP antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3F310
• Specificity: Recognizes human p300 and CBP(1). p300 and CBP differ by three noncontiguous residues within the 21aa epitope region. A difference that does not detectably affect the reactivity of C2098-03. Species Crossreactivity: monkey, mink, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C2098-03.

• Applicable Applications for anti-CREBBP antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot.; Dilution: Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.; Optimal dilutions to be determined by the researcher
• Immunogen: E1A affinity purified p300 protein. Cellular Localization; Nuclear. MW of Antigen; 300kD. Epitope; aa2071-2091.
• Positive Control: Tonsil
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot, human cells)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human tonasil stained with MBS631812 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded rat spleen stained with MBS631812 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells)

Related Product Information for anti-CREBBP antibody

p300 is a transcriptional adapter protein which is characterized by three cysteine-and histidine-rich regions and its C-terminus specifically binds the adenovirus E1A protein. p300, and p300-associated proteins, are components of TATAbinding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265kD nuclear protein designated CBP (for CREB-binding protein). CBP and p300 are homologous to each other.

Product Categories/Family for anti-CREBBP antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 3168627
• NCBI GeneID: 1387
• UniProt Accession #: O60424; Q4G0V0; Q4LE28; Q712H6; Q75MY6; Q92793
• Molecular Weight: 101,798 Da
• NCBI Official Full Name: CBP
• NCBI Official Synonym Full Names: CREB binding protein
• NCBI Official Symbol: CREBBP
• NCBI Official Synonym Symbols: CBP; RSTS; KAT3A
• NCBI Protein Information: CREB-binding protein; OTTHUMP00000159801
• UniProt Protein Name: CBP
• Protein Family: CREB-binding protein
• UniProt Gene Name: CBP
• UniProt Entry Name: O60424_HUMAN

NCBI Description

This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Uniprot Description

CBP: a protein acetyltransferase that can transcriptionally activate histones. Acetylates the NCOA3 coactivator. Binds specifically to phosphorylated CREB1 and enhances its transcriptional activity toward cAMP-responsive genes. Methylation of the KIX domain by CARM1 blocks association with CREB, blocking CREB signaling, and activating the apoptotic response. Found in a complex containing NCOA2, NCOA3, IKKA, IKKB, and IKBKG. Probably part of a complex with HIF1A and EP300. Interacts with the C-terminal region of CITED4. The TAZ-type 1 domain interacts with HIF1A. Interacts with MAF, SRCAP, CARM1, ELF3, MLLT7/FOXO4, N4BP2, NCOA1, NCOA3, NCOA6, PCAF, PELP1, PML, SMAD1, SMAD2, SMAD3, SPIB and TRERF1. Interacts with HTLV-1 Tax, p30II, and HIV-1 Tat. Interacts with KLF1; the interaction results in acetylation of KLF1 and enhancement of its transcriptional activity. Interacts with ZCCHC12. Interacts with DAXX; the interaction is dependent on CBP sumoylation and results in suppression of the transcriptional activiy via recruitment of HDAC2 to DAAX. Interacts with MTDH. Interacts with NFATC4. Interacts with MAFG; the interaction acetylates MAFG in the basic region and stimulates NFE2 transcriptional activity through increasing its DNA-binding activity. Interacts with IRF2; the interaction acetylates IRF2 and regulates its activity on the H4 promoter. Interacts via its N-terminus with the C-terminus of SS18L1. Interacts with MECOM. Chromosomal aberrations involving CBP may be a cause of acute myeloid leukemias. Known translocation partners include MYST3, MLL, and MYST4. MYST3-CBP fusion proteins may induce leukemia by inhibiting RUNX1-mediated transcription. Defects in CBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1), an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.

Protein type: Motility/polarity/chemotaxis; EC 2.3.1.48; Acetyltransferase; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; nuclear body; transcription factor complex; cytoplasm; nuclear chromatin; outer kinetochore of condensed chromosome; nucleus; histone acetyltransferase complex

Molecular Function: signal transducer activity; protein binding; MRF binding; histone acetyltransferase activity; zinc ion binding; p53 binding; acetyltransferase activity; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; viral reproduction; positive regulation of transcription, DNA-dependent; rhythmic process; germ-line stem cell maintenance; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; homeostatic process; regulation of transcription, DNA-dependent; response to hypoxia; positive regulation of interferon type I production; innate immune response; gene expression; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; embryonic digit morphogenesis; histone acetylation; N-terminal peptidyl-lysine acetylation; regulation of smoothened signaling pathway

Disease: Rubinstein-taybi Syndrome 1