Mouse Catenin, delta-1 (CTNND1) Monoclonal Antibody | anti-CTNND1 antibody

Catenin, delta-1 (CTNND1) (pTyr96)

Cat. Num. AAA439904

• Reactivity: Human, Rat. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: Catenin; delta-1 (CTNND1); Monoclonal Antibody; delta-1 (CTNND1) (pTyr96); Cadherin-associated Src substrate (CAS); Catenin (cadherin associated protein) delta 1; Catenin delta-1; CTNND1; p120 catenin; P120 CTN; p120(cas); p120(ctn); P120CAS; P120CTN; anti-CTNND1 antibody

• Host: Mouse
• Reactivity: Human, Rat. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 25a
• Specificity: The membrane associated protein pp120 Src substrate (p120 Catenin, p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src transformed cells or in response to growth factor stimulation. It shares structural similarity with the Drosophila Armadillo protein and the vertebrate beta-catenin and gamma-catenin proteins. Its characteristic Arm domain that is composed of 42-amino acid motif repeats evidences this similarity. In the cell, p120 Catenin is localized to the E-Cadherin/catenins cell adhesion complex. Like beta- and gamma-catenin, p120 Catenin directly associates with the cytoplasmic C-terminus of E-Cadherin via its Arm domain and may similarly interact with other Cadherins. It exists as four isoforms that range in size from 90-115kDa. Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered pp120 expression contributes to malignancy due to loss of functional cell adhesions. Multiple tyrosine residues (Y96, Y112, Y228, Y280, Y257, Y291, Y296, and Y302) in p120 Catenin are phosphorylated by Src and these phosphorylations may facilitate interaction with PTP1C/SHP-1 in response to EGF stimulation. Thus, p120 Catenin is an Arm domain protein that interacts with both cell adhesion molecules, such as cadherins and cell signaling molecules, such as PTP1C.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 968

• Applicable Applications for anti-CTNND1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml
• Immunogen: Mouse p120 Catenin (pY96)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 11q11
• Positive Control: Carcinoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded Mouse Kidney stained with CFTR Monoclonal Antibody (CFTR/1342).)

References

Reynolds, Daniel, McCrea et al.: "Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8333-42, 1994. Mariner, Anastasiadis, Keilhack et al.: "Identification of Src phosphorylation sites in the catenin p120ctn." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28006-13, 2001

NCBI and Uniprot Product Information

• NCBI GI #: 146231940
• NCBI GeneID: 1500
• NCBI Accession #: NP_001078927.1
• NCBI GenBank Nucleotide #: NM_001085458.1
• UniProt Accession #: O60716; O15088; O60713; O60714; O60715; O60935; Q6DHZ7; Q6RBX8; Q9UP71; Q9UP72; Q9UP73; A8K939
• Molecular Weight: 120kDa
• NCBI Official Full Name: catenin delta-1 isoform 1ABC
• UniProt Protein Name: Catenin delta-1
• Protein Family: Catenin
• UniProt Gene Name: CTNND1
• UniProt Synonym Gene Names: KIAA0384; CAS; p120(ctn)

Uniprot Description

Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (). Associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.