Mouse anti-Human carbonic anhydrase XII Monoclonal Antibody | anti-CA12 antibody

Anti-carbonic anhydrase XII Antibody, Mouse Monoclonal

Cat. Num. AAA8103377

• Gene Names: CA12; CAXII; HsT18816
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: carbonic anhydrase XII; Monoclonal Antibody; Anti-carbonic anhydrase XII Antibody; Mouse Monoclonal; Carbonic Anhydrase XII Antibody; Mouse MAb; Anti-CA12 Antibody; Anti-CAXII Antibody; Anti-FLJ20151 Antibody; Anti-HsT18816 Antibody; anti-CA12 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 11
• Specificity: Human Carbonic Anhydrase XII
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CA12 antibody: Flow Cytometry (FC/FACS/FCM)
• Application Notes: FC/FACS/FCM: 1:25-1:100
• Immunogen: Recombinant Human Carbonic Anhydrase XII protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase XII (rh Carbonic Anhydrase XII). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human CA12 expression on MCF-7 cells. Cells were stained with purified anti-Human CA12, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-CA12 antibody

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning alpha-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

References

Sahin, U. et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11810-11813. Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596-12601. Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899-16903. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257-262. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.

NCBI and Uniprot Product Information

• NCBI GI #: 4502515
• NCBI GeneID: 771
• NCBI Accession #: NP_001209.1
• NCBI GenBank Nucleotide #: NM_001218.3
• UniProt Accession #: O43570; Q53YE5; Q9BWG2
• Molecular Weight: 39,451 Da
• NCBI Official Full Name: carbonic anhydrase 12 isoform 1
• NCBI Official Synonym Full Names: carbonic anhydrase XII
• NCBI Official Symbol: CA12
• NCBI Official Synonym Symbols: CAXII; HsT18816
• NCBI Protein Information: carbonic anhydrase 12; CA-XII; carbonic dehydratase; carbonate dehydratase XII; tumor antigen HOM-RCC-3.1.3
• UniProt Protein Name: Carbonic anhydrase 12
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA12
• UniProt Synonym Gene Names: CA-XII
• UniProt Entry Name: CAH12_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CA12: Reversible hydration of carbon dioxide. Homodimer. Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA), benzenesulfonamide and derivatives (4-carboxyethylbenzene-sulfonamide, 4- carboxyethylbenzene-sulfonamide ethyl ester, 4-(acetyl-2- aminoethyl)benzene-sulfonamide, 4-aminoethylbenzene-sulfonamide) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Energy Metabolism - nitrogen; EC 4.2.1.1; Membrane protein, integral; Lyase

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: integral to membrane; plasma membrane

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process; chloride ion homeostasis

Disease: Hyperchlorhidrosis, Isolated