Mouse anti-Human, Rat Calponin-1 Monoclonal Antibody | anti-CNN1 antibody

Calponin-1 (Smooth Muscle Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439276

• Gene Names: CNN1; SMCC; Sm-Calp; HEL-S-14
• Reactivity: Human, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Calponin-1; Monoclonal Antibody; Calponin-1 (Smooth Muscle Marker) Mouse Monoclonal Antibody; Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; CNN1; Cnn1; Sm Calp; SMCC; anti-CNN1 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CALP
• Specificity: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 297

• Applicable Applications for anti-CNN1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: Crude human uterus extract
• Hu-Chromosome Location: 19p13.2
• Positive Control: Myoepithelial cells in breast ducts
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Monoclonal Antibody (CALP).)

References

Frid MG, et al. Phenotypic changes of human smooth muscle cells during development: Late expression of heavy caldesmon and calponin. Dev Biol 1992; 153:18

NCBI and Uniprot Product Information

• NCBI GI #: 21361120
• NCBI GeneID: 1264
• NCBI Accession #: NP_001290.2
• NCBI GenBank Nucleotide #: NM_001299.5
• UniProt Accession #: P51911; O00638; Q15416; Q8IY93; Q99438; B2R868; B4DUX6
• Molecular Weight: 34kDa
• NCBI Official Full Name: calponin-1 isoform 1
• NCBI Official Synonym Full Names: calponin 1
• NCBI Official Symbol: CNN1
• NCBI Official Synonym Symbols: SMCC; Sm-Calp; HEL-S-14
• NCBI Protein Information: calponin-1
• UniProt Protein Name: Calponin-1
• Protein Family: Calponin
• UniProt Gene Name: CNN1
• UniProt Entry Name: CNN1_HUMAN

Uniprot Description

calponin 1: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family.

Protein type: Motility/polarity/chemotaxis; Contractile; Actin-binding; Myosin-binding

Chromosomal Location of Human Ortholog: 19p13.2-p13.1

Cellular Component: cytoskeleton; focal adhesion

Biological Process: regulation of smooth muscle contraction