Mouse anti-Human Calgranulin B Monoclonal Antibody

Anti-Human Calgranulin B, PE (Clone CF-557) (mouse IgG1)

Cat. Num. AAA520022

• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: Calgranulin B; Monoclonal Antibody; Anti-Human Calgranulin B; PE (Clone CF-557) (mouse IgG1); Anti-Human Calgranulin B Monoclonal Antibody; anti-Calgranulin B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: CF-557
• Specificity: Calgranulin B (Binds to human Calgranulin B)
• Form/Format: PE
• Concentration: 0.1 mg/ml (varies by lot)
• Sequence Length: 118

• Applicable Applications for anti-Calgranulin B antibody: Immunohistochemistry (IHC)
• Immunogen: Purified granulocyte antigen of human origin
• Donor: human leukocytes
• Fusion Partner: NSO Mouse Myeloma Partner
• Presentation: Purified IgG buffered in PBS and 0.02% NaN3. (Purified from ascitic fluid via Protein G Chromatography).
• Preparation and Storage: For all formats, store at 4 degree C. For long term storage (Ascites, Purified, Biotin, FITC), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles. Do not freeze PE conjugates.

Testing Data

Related Product Information for anti-Calgranulin B antibody

Calgranulin B, also known as MRP-14 and S100A9 is a member of the S100 family of proteins containing 2 EF hand (alpha helix, turn, alpha helix structure) calcium binding motifs. S100 proteins are localized in the cytoplasm and /or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. MRP-14 (S100A9) forms a heterodimeric complex with MRP-8 (S100A8) in the cytosol of monocyte and neutrophil cell types circulating in peripheral blood. Calgranulin B is found in elevated levels in the serum of cystic fibrosis cases and is also expressed in the skin of patients with psoriasis, eczematous dermatitis and squamous cell carcinoma. Calgranulin B is also expressed on macrophages in acute inflamed tissues (peridontitis, contact excema). We also sell an ascites purified anti-human Calgranulin B monoclonal antibody, as well as its respective Biotin, FITC and PE conjugates. Reported applications of this antibody include flow cytometry, IF, IHC and Western blots.

NCBI and Uniprot Product Information

• NCBI GI #: 3661599
• NCBI Official Full Name: calgranulin B

Uniprot Description

S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants (). The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, NXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif ().