Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 30ug/mL Mouse Anti-Human S100 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Mouse anti-Human Calcium Binding Protein (S100) Monoclonal Antibody | anti-S100 antibody
HRP-Linked Monoclonal Antibody to S100 Calcium Binding Protein (S100)
Please refer to the vial label for the specific concentration. (varies by lot)
IHC: 5-30ug/mL
ICC: 5-30ug/mL
Optimal working dilutions must be determined by end user.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 30ug/mL Mouse Anti-Human S100 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 30ug/mL Mouse Anti-Human S100 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot; Sample: Human HT1080 cell lysate; Primary Ab: 2ug/mL Mouse Anti-Human S100 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot; Sample: Human HT1080 cell lysate; Primary Ab: 2ug/mL Mouse Anti-Human S100 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]
Uniprot Description
S100A1: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. Belongs to the S-100 family.
Protein type: Calcium-binding; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: neuron projection; protein complex; sarcoplasmic reticulum; M band; Z disc; nucleus
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; calcium ion binding; calcium-dependent protein binding; ATPase binding
Biological Process: substantia nigra development; negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction
Research Articles on S100
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Product Notes
The S100 s100a1 (Catalog #AAA2124722) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HRP-Linked Monoclonal Antibody to S100 Calcium Binding Protein (S100) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calcium Binding Protein (S100) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-3ug/mL IHC: 5-30ug/mL ICC: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the S100 s100a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calcium Binding Protein (S100), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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