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Immunohistochemistry (IHC) (Anti-CA3 / Carbonic Anhydrase III antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Mouse anti-Human CA3 / Carbonic Anhydrase III Monoclonal Antibody | anti-CA3 antibody

Mouse Monoclonal [clone 4A12-1A3] (IgG1,k) to Human CA3 / Carbonic Anhydrase III

Gene Names
CA3; Car3; CAIII
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
CA3 / Carbonic Anhydrase III; Monoclonal Antibody; Mouse Monoclonal [clone 4A12-1A3] (IgG1; k) to Human CA3 / Carbonic Anhydrase III; Anti-CA3 / Carbonic Anhydrase III Antibody (clone 4A12-1A3) IHC-plus; CA3; Carbonate dehydratase III; Carbonic anhydrase 3; Carbonic anhydrase III; CA-III; CAIII; Car3; Human CA3; Carbonic Anhydrase III; anti-CA3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4A12-1A3
Specificity
Human Carbonic Anhydrase III
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Concentration
0.22 mg/ml (varies by lot)
Sequence Length
260
Applicable Applications for anti-CA3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Target Species
Human
Immunogen Description
CA3 (AAH04897, 1 a.a. ~ 261 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
CA3 / Carbonic Anhydrase III antibody was raised against cA3 (AAH04897, 1 a.a. ~ 261 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-CA3 / Carbonic Anhydrase III antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-CA3 / Carbonic Anhydrase III antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of CA3 expression in K-562 by CA3 monoclonal antibody (M02), clone 4A12-1A3.)

Western Blot (WB) (Western blot of CA3 expression in K-562 by CA3 monoclonal antibody (M02), clone 4A12-1A3.)

ELISA (EIA)

(Detection limit for recombinant GST tagged CA3 is approximately 1 ng/ml as a capture antibody.)

ELISA (EIA) (Detection limit for recombinant GST tagged CA3 is approximately 1 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
761
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,557 Da
NCBI Official Full Name
carbonic anhydrase 3
NCBI Official Synonym Full Names
carbonic anhydrase III, muscle specific
NCBI Official Symbol
CA3
NCBI Official Synonym Symbols
Car3; CAIII
NCBI Protein Information
carbonic anhydrase 3; CA-III; HEL-S-167mP; carbonate dehydratase III; epididymis secretory sperm binding protein Li 167mP
UniProt Protein Name
Carbonic anhydrase 3
Protein Family
UniProt Gene Name
CA3
UniProt Synonym Gene Names
CA-III
UniProt Entry Name
CAH3_HUMAN

NCBI Description

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]

Uniprot Description

CA3: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Lyase; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding; nickel ion binding

Biological Process: response to ethanol; bicarbonate transport; response to oxidative stress; one-carbon compound metabolic process

Research Articles on CA3

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Product Notes

The CA3 ca3 (Catalog #AAA246445) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 4A12-1A3] (IgG1,k) to Human CA3 / Carbonic Anhydrase III reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CA3 / Carbonic Anhydrase III can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (5 ug/ml), WB Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein. Researchers should empirically determine the suitability of the CA3 ca3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CA3 / Carbonic Anhydrase III, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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