Mouse Lanosterol 14-alpha demethylase (CYP51A1) ELISA Kit | CYP51A1 elisa kit

Mouse Lanosterol 14-alpha demethylase (CYP51A1) ELISA Kit

Cat. Num. AAA246445

• Gene Names: CYP51A1; LDM; CP51; CYP51; CYPL1; P450L1; P450-14DM
• Reactivity: Mouse
• Synonyms: Lanosterol 14-alpha demethylase (CYP51A1); N/A; Mouse Lanosterol 14-alpha demethylase (CYP51A1) ELISA Kit; CYP51A1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CYP51A1. No significant cross-reactivity or interference between CYP51A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP51A1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CYP51A1 elisa kit

Principle of the Assay: CYP51A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CYP51A1 antibody and an CYP51A1-HRP conjugate. The assay sample and buffer are incubated together with CYP51A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP51A1 concentration since CYP51A1 from samples and CYP51A1-HRP conjugate compete for the anti-CYP51A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP51A1 from the sample, fewer sites are left to bind CYP51A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP51A1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This CYP51A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CYP51A1. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 225903399
• NCBI GeneID: 1595
• NCBI Accession #: NP_001139624.1
• NCBI GenBank Nucleotide #: NM_001146152.1
• Molecular Weight: 46,312 Da
• NCBI Official Full Name: lanosterol 14-alpha demethylase isoform 2
• NCBI Official Synonym Full Names: cytochrome P450, family 51, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP51A1
• NCBI Official Synonym Symbols: LDM; CP51; CYP51; CYPL1; P450L1; P450-14DM
• NCBI Protein Information: lanosterol 14-alpha demethylase; CYPLI; cytochrome P450LI; cytochrome P45014DM; cytochrome P450 51A1; cytochrome P450-14DM; lanosterol 14-alpha-demethylase; cytochrome P450, 51 (lanosterol 14-alpha-demethylase)
• UniProt Protein Name: Lanosterol 14-alpha demethylase
• UniProt Gene Name: CYP51A1
• UniProt Synonym Gene Names: CYP51; LDM; Cytochrome P45014DM
• UniProt Entry Name: CP51A_HUMAN