Rat anti-Mouse C3aR Monoclonal Antibody | anti-C3aR antibody
C3aR, Mouse, mAb 14D4
Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
C3aR; Monoclonal Antibody; Mouse; mAb 14D4; anti-C3aR antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a
Clone Number
14D4
Purity/Purification
Protein G
Form/Format
0.2um filtered in PBS + 0.1% BSA + 0.02% NaN3
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-C3aR antibody
Imunohistochemistry-Frozen sections (IHC-F), Flow Ctometry (FC/FACS)
Application Notes
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Flow Cytometry (FC): Antibody 14D4 stains the extracellular domain of C3aR when RBL-2H3 transfectants and human monocytes are used. Primary antibody concentrations were 5ug/ml. (Ref.1)
Immunohistochemistry-Frozen (IHC-F): Tissue sections were fixed in acetone. To block unspecific binding of Abs, sections were incubated with PBS and heat aggregated Human IgG at 300 ug/ml.
Flow Cytometry (FC): Antibody 14D4 stains the extracellular domain of C3aR when RBL-2H3 transfectants and human monocytes are used. Primary antibody concentrations were 5ug/ml. (Ref.1)
Immunohistochemistry-Frozen (IHC-F): Tissue sections were fixed in acetone. To block unspecific binding of Abs, sections were incubated with PBS and heat aggregated Human IgG at 300 ug/ml.
Immunogen
RBL-2H3 transfectants expressing mouse C3aR
Preparation and Storage
Product should be stored at 4°C. The exact expiry date is indicated on the label.
Related Product Information for anti-C3aR antibody
The monoclonal antibody 14D4 reacts with mouse C3aR, which is a member of the rhodopsin superfamily of 7-transmembrane G protein-coupled receptors, with a molecular weight of approximately 54 kDa. Expression of C3aR has been demonstrated on a wide variety of immune cells, including monocytes, macrophages, dendritic cells, neutrophils, basophils, eosinophils, mast cells, T lymphocytes and B lymphocytes. In addition, C3aR is found on cells of the central nervous system, lungs and kidney. In the course of complement activation C3aR functions as the cell surface receptor for C3a, which is the C-terminal 77 amino acid cleavage product of C3. C3a, possesses both anaphylatoxic and immunoregulatory properties, such as smooth muscle contraction, histamine release from mast cells, and enhanced vascular permeability. In addition, C3a induces respiratory burst in neutrophils and has chemotactic properties for eosinophils and mast cells. Moreover, C3a causes release of key cytokines from multiple cell types, including IL-1beta, TNF-alpha, IL-6 and IL-8. A role for C3aR has been implicated in several murine disease models. It was shown that C3aR inhibition reduces neurodegeneration in experimental lupus, whereas in a murine model of allergic airway disease, deletion of the C3a receptor protects against the changes in lung physiology seen after allergen challenge. Finally, it was shown that deletion of C3aR is protective in myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis.
References
Kiafard Z, et al; Use of monoclonal antibodies to assess expression of anaphylatoxin receptors in tubular epithelial cells of human, murine and rat kidneys. Immunobiology 2007, 212: 129.
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Product Notes
The C3aR (Catalog #AAA584674) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The C3aR, Mouse, mAb 14D4 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's C3aR can be used in a range of immunoassay formats including, but not limited to, Imunohistochemistry-Frozen sections (IHC-F), Flow Ctometry (FC/FACS). Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Flow Cytometry (FC): Antibody 14D4 stains the extracellular domain of C3aR when RBL-2H3 transfectants and human monocytes are used. Primary antibody concentrations were 5ug/ml. (Ref.1) Immunohistochemistry-Frozen (IHC-F): Tissue sections were fixed in acetone. To block unspecific binding of Abs, sections were incubated with PBS and heat aggregated Human IgG at 300 ug/ml. . Researchers should empirically determine the suitability of the C3aR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C3aR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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