Rat anti-Mouse C3 Monoclonal Antibody | anti-C3 antibody

C3, Mouse, mAb 11H9

Cat. Num. AAA584047

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: C3; Monoclonal Antibody; Mouse; mAb 11H9; Complement component C3; anti-C3 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 825

• Applicable Applications for anti-C3 antibody: F, Flow Cytometry (FC/FACS), Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Application Notes: IA: Antibody 11H9 was used as coating 0.5 ug/well in PBS.; Application Use: For Western blotting, flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Immunogen: C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1)
• Positive Control: MPL-lpr kidney cellsF:- As positive control brain sections of APPQ mice were used and as negative control isotype matched material
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-C3 antibody

Product Description: The monoclonal antibody 11H9 recognizes mouse complement component C3 and its activation products, C3b, iC3b, C3d and C3dg. The complement system is an important part of the humoral response in innate immunity, consisting of three different pathways. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. The complement factor C3 consists of an alpha- and a beta-chain.
The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis, and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported.
Proteolysis by certain enzymes results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. The monoclonal antibody 11H9 recognizes both intact C3 and its cleaved products C3b, iC3b, C3d and C3dg. These activation products are present in acute as well as chronic inflammatory conditions. In chronic inflammatory condition, primarily the C3dg product resides at the place of inflammation (C3c being cleared) which is recognized by antibody 11H9. The chronic processing/activation of C3 is taking place at a lower level, which would reduce detection of the C3 fragments C3b, iC3b, and C3c.

References

1. Kremmer, E et al; Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4. Hybridoma 1990, 9: 309 2. Pan, W et al; CR3 (CD11b/CD18) is the major macrophage receptor for IgM antibody-mediated phagocytosis of African trypanosomes: Diverse effect on subsequent synthesis of tumor necrosis factor a and nitric oxide. Microb Infect 2006, 8: 1209
3. Zhou, J et al; Complement C3 and C4 expression in C1q sufficient and deficient mouse models of Alzheimer's disease. J Neurochem 2008, 106: 2080

NCBI and Uniprot Product Information

• NCBI GI #: 24943136
• NCBI GeneID: 955871
• NCBI Accession #: NP_733898.1
• NCBI GenBank Nucleotide #: NC_004367.1
• Molecular Weight: 92,707 Da
• NCBI Official Full Name: C3
• NCBI Official Symbol: C3
• NCBI Protein Information: C3
• UniProt Protein Name: C3
• Protein Family: Complement C3
• UniProt Gene Name: C3
• UniProt Entry Name: Q993G5_9GAMA