Rabbit anti-Human C1s  Monoclonal Antibody | anti-C1s  antibody

Rabbit anti Human C1s  Monoclonal Antibody

Cat. Num. AAA2543886

• Gene Names: C1S; EDSPD2
• Reactivity: Human
• Applications: ELISA
• Synonyms: C1s ; Monoclonal Antibody; Rabbit anti Human C1s Monoclonal Antibody; anti-C1s antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 688

• Applicable Applications for anti-C1s antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human C1S. The detection limit for Human C1S is approximately 0.00245 ng/well.
• Immunogen: Recombinant Human Complement C1s protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-C1s  antibody

Complement is an integral component of the adaptive and innate immune systems and represents one of the major effector systems for the immune responses. The classical complement pathway is triggered by C1, a complex composed of the binding protein C1q and two proenzymes, C1r and C1s. Upon binding of IgG to the head of C1q, C1r undergoes autoactivation and in turn cleaves and activates C1s. C1r and C1s, the proteases responsible for activation and proteolytic activity of the C1 complex of complement, share similar overall structural organizations featuring five nonenzymic protein modules (two CUB modules surrounding a single EGF module, and a pair of CCP modules) followed by a serine protease domain. Besides highly specific proteolytic activities, both proteases exhibit interaction properties associated with their N-terminal regions. In contrast, C1r and C1s widely differ from each other by their glycosylation patterns: both proteins contain Asn-linked carbohydrates, but four glycosylation sites are present on C1r, and only two on C1s. As a highly specific serine protease, C1s executes the catalytic function of the C1 complex: the cleavage of C4 and C2, and thus instigates a sequence of activation steps of other components of the complement system, culminating in the formation of the membrane attack complex which induces cell lysis. Like other complement serine proteases C1s has restricted substrate specificity and it is engaged into specific interactions with other subcomponents of the complement system. The only other protein known to interact with C1s physiologically is SerpinC1, an inhibitor of serine protease, which inhibits C1s activity and thus plays a regulatory role in controlling the function of C1s enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 41393602
• NCBI GeneID: 716
• NCBI GenBank Nucleotide #: NM_201442.3
• Molecular Weight: 76,684 Da
• NCBI Official Full Name: complement C1s subcomponent isoform 1 preproprotein
• NCBI Official Synonym Full Names: complement C1s
• NCBI Official Symbol: C1S
• NCBI Official Synonym Symbols: EDSPD2
• NCBI Protein Information: complement C1s subcomponent
• UniProt Protein Name: Complement C1s subcomponent
• UniProt Gene Name: C1S
• UniProt Entry Name: C1S_HUMAN

NCBI Description

This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. [provided by RefSeq, Mar 2009]

Uniprot Description

C1S: C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4. Defects in C1S are the cause of complement component C1s deficiency (C1SD). A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.42; Protease

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: extracellular region

Molecular Function: identical protein binding; protein binding; serine-type endopeptidase activity

Biological Process: complement activation; complement activation, classical pathway; complement activation, lectin pathway

Disease: Complement Component C1s Deficiency; Ehlers-danlos Syndrome, Periodontal Type, 2