c-myc epitope tag Recombinant Antibody | cmyc recombinant antibody
Anti-c-myc epitope tag [9E10]
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Purified by Immobilized Metal Affinity Chromatography
Synonyms
c-myc epitope tag; Monoclonal Recombinant Antibody; Anti-c-myc epitope tag [9E10]; Proto-oncogene c-Myc; Transcription factor p64; Class E basic helix-loop-helix protein 39; BHLHE39; cmyc recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse F(ab)2, AbFab2 His-Tagged, kappa
Clone Number
9E10
Specificity
Epitope sequence, EQKLISEEDL, located in aa 410-419 of human c-myc protein.
Purity/Purification
Purified by Immobilized Metal Affinity Chromatography
Applicable Applications for cmyc recombinant antibody
Immunohistochemistry (IHC) Formalin/Paraffin, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Immunogen
A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc.
Antigen
c-myc epitope tag
Antigen Description
A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc.
Buffer Composition
PBS only with 0.02% Proclin 300.
Heavy Chain Modification
AbFab2 His-Tagged
Chimeric Use Statement
This is a recombinant mouse Fab2 fragment, based on the original mouse IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Publication Note
Describes the making of the antibody, shows it recognises a 62kDa c-myc protein in c-myc overexpressing cell lines (Western blot).
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for cmyc recombinant antibody
Recombinant monoclonal antibody to c-myc epitope tag. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 9E10. This antibody is in our proprietary AbFab2 recombinant F(ab'2) format - based on Mouse IgG1 sequence with a short dimerization domain to improve stability and a his tag.
Product Categories/Family for cmyc recombinant antibody
References
Evan GI, Lewis GK, Ramsay G, Bishop JM. Mol Cell Biol. 1985 Dec;5(12):3610-6. PMID:3915782
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Product Notes
The cmyc (Catalog #AAA488216) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-c-myc epitope tag [9E10] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-myc epitope tag can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin/Paraffin, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the cmyc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-myc epitope tag, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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