Mouse anti-Human C/EBP Beta / CEBPB Monoclonal Antibody | anti-CEBPB antibody

Mouse Monoclonal [clone 21A1] (IgG) to Human C/EBP Beta / CEBPB

Cat. Num. AAA245066

• Gene Names: CEBPB; TCF5; IL6DBP; NF-IL6; C/EBP-beta
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation
• Purity: Protein G Purified
• Synonyms: C/EBP Beta / CEBPB; Monoclonal Antibody; Mouse Monoclonal [clone 21A1] (IgG) to Human C/EBP Beta / CEBPB; Anti-C/EBP Beta / CEBPB Antibody (aa39-78; clone 21A1) IHC-plus; CEBPB; C/EBP-beta; C/EBP beta; CRP2; LAP; Liver activator protein; Nuclear factor NF-IL6; IL6DBP; TCF5; NFIL6; TCF-5; Transcription factor 5; NF-IL6; Human C/EBP Beta; anti-CEBPB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 21A1
• Specificity: Reported to recognize an epitope on NF-IL6 within amino acids 39-78
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.4. No preservative added.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 345

• Applicable Applications for anti-CEBPB antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IHC-P (10 ug/ml), IP, WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Purified human CEBPB / CEBP Beta.
• Immunogen: C/EBP Beta / CEBPB antibody was raised against purified human CEBPB / CEBP Beta.
• Antigen Modification: aa39-78
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CEBPB / CEBP Beta antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 28872796
• NCBI GeneID: 1051
• NCBI Accession #: NP_005185.2
• NCBI GenBank Nucleotide #: NM_005194.3
• UniProt Accession #: P17676; Q96IH2; Q9H4Z5; A8K671
• Molecular Weight: 15,938 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein beta isoform a
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), beta
• NCBI Official Symbol: CEBPB
• NCBI Official Synonym Symbols: TCF5; IL6DBP; NF-IL6; C/EBP-beta
• NCBI Protein Information: CCAAT/enhancer-binding protein beta; nuclear factor NF-IL6; transcription factor 5; nuclear factor of interleukin 6; interleukin 6-dependent DNA-binding protein
• UniProt Protein Name: CCAAT/enhancer-binding protein beta
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPB
• UniProt Synonym Gene Names: TCF5; C/EBP beta; LAP; LIP; TCF-5
• UniProt Entry Name: CEBPB_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]

Uniprot Description

C/EBP-beta: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-alpha, -delta and -gamma. Involved in immune and inflammatory responses. Specifically binds to regulatory regions of genes encoding IL-6, other cytokines and several acute-phase proteins. There are two forms of C/EBPbeta, the 38kDa liver activating protein (LAP) and the 20kDa liver inhibitory protein (LIP) which may be products of alternative translation. LAP is a transcriptional activator while LIP may inhibit C/EBPbeta transcriptional activity. Phosphorylated and activated by ERK1/2.

Protein type: DNA-binding; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm; nuclear matrix; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity; transcription factor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; regulation of interleukin-6 biosynthetic process; mammary gland epithelial cell proliferation; response to lipopolysaccharide; neuron differentiation; positive regulation of osteoblast differentiation; regulation of transcription, DNA-dependent; brown fat cell differentiation; acute-phase response; positive regulation of fat cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; negative regulation of transcription, DNA-dependent; inflammatory response