BoTox-A Clone 230F9 Monoclonal Antibody | anti-botA antibody

Anti-Botulism Toxin-A Monoclonal Antibody 230F9

Cat. Num. AAA350002

• Applications: Immunofluorescent Staining of Neuro2A Cells
• Synonyms: BoTox-A Clone 230F9; Monoclonal Antibody; Anti-Botulism Toxin-A Monoclonal Antibody 230F9; BoTox-A (Clone 230F9); anti-botA antibody

• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone Number: 230F9
• Specificity: Polypeptide ::: % Cross Reactivity; BoTox-A (1280-1292) ::: 100; BoTox-A ::: 100
• Form/Format: Supplied as Sterile Culture Supernatant
• Sequence Length: 1296

• Applicable Applications for anti-botA antibody: Immunofluorescent Staining of Neuro2A Cells
• Application Notes: Immunofluorescent: This monoclonal antibody containing culture supernatant has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-A at a dilution of 1:50 using an indirect immunocytochemical staining procedure. Following the treatment. the cells were washed x 4. fixed in neutral buffered formalin for 10 min. permeablized and blocked. They were reacted for 60 minutes with the culture supernatant diluted 1:50. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescent microscopy.
• Inhibition: Inhibition of BoTox-A binding to Neuro2A Cells
• Inhibition Note: This monoclonal antibody containing culture supernatant has been found to inhibit the binding of BoTox-A to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin. and prior incubation of the antibody with the synthetic peptide immunogen has been shown to eliminate the inhibition of cellular binding by the antibody.

Related Product Information for anti-botA antibody

This sterile filtered culture supernatant contains mouse monoclonal antibody 230F9. a mouse IgG class MAb. raised against a synthetic peptide analogue of the carboxyl terminal region of BoTox-A. This monoclonal antibody has been found to bind to intact whole BoTox-A by ELISA. RIA and cell staining techniques. This monoclonal antibody was tested for recognition of other BoTox isoforms by ELISA and immunocytochemical techniques. Each vial contains 1.0 ml of culture supernatant

Product Categories/Family for anti-botA antibody

BoTox and BoTox Substrate Antibodies and Kits

NCBI and Uniprot Product Information

• NCBI GI #: 399133
• NCBI Accession #: P10845
• NCBI GenBank Nucleotide #: P10845
• UniProt Accession #: P10845; P01561; P18639
• Molecular Weight: 149,454 Da
• NCBI Official Full Name: Botulinum neurotoxin type A
• NCBI Official Symbol: botA
• UniProt Protein Name: Botulinum neurotoxin type A
• Protein Family: Botulinum neurotoxin
• UniProt Gene Name: botA
• UniProt Synonym Gene Names: atx; bna
• UniProt Entry Name: BXA1_CLOBO

Uniprot Description

Function: Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.

Catalytic activity: Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates.

Cofactor: Binds 1 zinc ion per subunit.

Subunit structure: Disulfide-linked heterodimer of a light chain (L) and a heavy chain (H).

Subcellular location: Botulinum neurotoxin A light chain: Secreted. Host cytoplasm › host cytosol. Botulinum neurotoxin A heavy chain: Secreted. Host cell junction › host synapse › host presynaptic cell membrane; Multi-pass membrane protein

Potential.

Pharmaceutical use: Available under the name BOTOX (Allergan) for the treatment of strabismus and blepharospasm associated with dystonia and cervical dystonia. Also used for the treatment of hemifacial spasm and a number of other neurological disorders characterized by abnormal muscle contraction.

Miscellaneous: There are seven antigenically distinct forms of botulinum neurotoxin: Types A, B, C1, D, E, F, and G.

Sequence similarities: Belongs to the peptidase M27 family.