Mouse Bleomycin Hydrolase Monoclonal Antibody | anti-BLMH antibody

Bleomycin Hydrolase (BH, BLMH, BMH)

Cat. Num. AAA601898

• Gene Names: BLMH; BH; BMH
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Bleomycin Hydrolase; Monoclonal Antibody; Bleomycin Hydrolase (BH; BLMH; BMH); Anti -Bleomycin Hydrolase (BH; anti-BLMH antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 8C32
• Specificity: Recognizes human Bleomycin Hydrolase.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-BLMH antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blotting: 1:1000-1:2000
• Immunogen: Partial sequence of recombinant full-length protein to human Bleomycin Hydrolase
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-BLMH antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4557367
• NCBI GeneID: 642
• NCBI Accession #: NP_000377.1
• NCBI GenBank Nucleotide #: NM_000386.3
• UniProt Accession #: Q13867; Q53F86; Q9UER9; B2R796
• Molecular Weight: 52,562 Da
• NCBI Official Full Name: bleomycin hydrolase
• NCBI Official Synonym Full Names: bleomycin hydrolase
• NCBI Official Symbol: BLMH
• NCBI Official Synonym Symbols: BH; BMH
• NCBI Protein Information: bleomycin hydrolase; BLM hydrolase
• UniProt Protein Name: Bleomycin hydrolase
• Protein Family: Bleomycin hydrolase
• UniProt Gene Name: BLMH
• UniProt Entry Name: BLMH_HUMAN

NCBI Description

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq]

Uniprot Description

BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.40; Protease

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; carboxypeptidase activity; cysteine-type endopeptidase activity; aminopeptidase activity; cysteine-type peptidase activity

Biological Process: response to drug; antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; response to toxin; proteolysis

Disease: Alzheimer Disease