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Mouse anti-Human Beta1 Integrin Monoclonal Antibody

Beta1 Integrin, Human, mAb BV7, FITC

Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Synonyms
Beta1 Integrin; Monoclonal Antibody; Human; mAb BV7; FITC; CD29; fibronectin receptor subunit beta; GPIIA; integrin VLA-4 subunit beta; anti-Beta1 Integrin antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Form/Format
1 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Applicable Applications for anti-Beta1 Integrin antibody
Flow Cytometry (FC/FACS), Immunoassay (IA), Western Blot (WB)
Application Notes
Application Notes: FC: Antibody BV7 stains the extracellular domain of beta-1 integrin. HUVEC cells were fixed in 4% paraformaldehyde before staining. Mouse IgG1 isotype was used as negative.
Application Use: For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Conjugate
FITC
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-Beta1 Integrin antibody
Product Description: Monoclonal antibody BV7 binds to human beta1 integrin chain present on tumor cells. BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.
References
Martin-Padura, I et al; A novel mechanism of colon carcinoma cell adhesion to the endothelium triggered by beta1 integrin chain. J Biol Chem 1994, 269: 6124

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Product Notes

The Beta1 Integrin (Catalog #AAA584405) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Beta1 Integrin, Human, mAb BV7, FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Beta1 Integrin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoassay (IA), Western Blot (WB). Application Notes: FC: Antibody BV7 stains the extracellular domain of beta-1 integrin. HUVEC cells were fixed in 4% paraformaldehyde before staining. Mouse IgG1 isotype was used as negative. Application Use: For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the Beta1 Integrin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta1 Integrin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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