Mouse anti-Human Beta1 Integrin Monoclonal Antibody

Beta1 Integrin, Human, mAb BV7

Cat. Num. AAA584125

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: Beta1 Integrin; Monoclonal Antibody; Human; mAb BV7; CD29; fibronectin receptor subunit beta; -integrin VLA-4 subunit beta; anti-Beta1 Integrin antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)

• Applicable Applications for anti-Beta1 Integrin antibody: Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Application Notes: FC: Antibody BV7 stains the extracellular domain of beta-1 integrin. HUVEC cells were fixed in 4% paraformaldehyde before staining. Mouse IgG1 isotype was used as negative.; Application Use: For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of beta1 integrin to be inactivated.
• Immunogen: Human umbilical vein EC
• Positive Control: HT-29 colon carcinoma, Endothelial cells
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-Beta1 Integrin antibody

Product Description: The monoclonal antibody BV7 recognizes human Ÿ1-integrin. Beta-1 integrin is a ubiquitously expressed ~89 kDa type I transmembrane protein functioning as receptor when heterodimerized with one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes, encoding multiple beta-integrins via alternative splicing. Ligand-recognition depends on the composition of the heterodimer: either collagen, fibronectin, VCAM1, laminin, cytotactin, osteopontin, epiligrin, thrombospondin and CSPG4 can bind to the integrin-complex. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha7, beta1-integrin regulates cell adhesion and laminin matrix deposition. BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. It is involved in promoting endothelial cell motility and angiogenesis. Furthermore, beta1-integrin plays a mechanistic adhesive role during telophase, and is required for the successful completion of cytokinesis. Upon activation integrins in general, including beta1-integrin, are known to exhibit global structural rearrangements and exposure of ligand binding sites. beta1-integrin modulation is of importance in tissue repair and regeneration. In cultured primary hippocampal neurons, astrocytes and tissues, cell surface expression of amyloid beta fibrils (key hallmark of Alzheimers disease) selectively co-localized with beta1-integrin. Preincubation of cells with antibodies against beta1-integrin, as well as alpha1-integrin, greatly enhanced amyloid beta-induced apoptosis, indicating a protective role for integrins in apoptosis. The monoclonal antibody BV7 does not recognize alpha5beta1 complex and not the cytoplasmic part of the beta1-subunit. Monoclonal antibody BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC- 1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.

References

1. Martin-Padura, I et al; A novel mechanism of colon carcinoma cell adhesion to the endothelium triggered by Ÿ1 integrin chain. J Biol Chem 1994, 269: 6124
2. Palmieri, D et al;Trimer carboxyl propeptide of collagen I produced by mature osteoblasts is chemotactic for endothelial cells. J Biol Chem 2000, 275: 32658
3. Martel, V et al; Talin controls exit of the integrin alpha5beta1 from an early compartment of the secretory pathway. J Cell Sci 2000, 113: 1951
4. Hofmann, G et al; HERG K+ channels activation during beta1 integrin-mediated adhesion to fibronectin induces an up-regulation of alphavbeta3 integrin in the preosteoclastic lekemia cell line FLG29.1. J Biol Chem 2001, 276: 4923
5. Bozzo, C et al; Involvement of beta1 integrin in betaAP-induced apoptosis in human neuroblastoma cells. Mol Cell Neurosci 2004, 25: 1