Western Blot (WB)
(Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-beta-Adaptin (N-terminal region) antibody at 1:500 (lane 1) and 1:2000 (lane 2).)
Mouse beta-Adaptin Monoclonal Antibody | anti-AP1B1 antibody
beta-Adaptin (N-terminal region) Antibody
Gene Names
AP1B1; ADTB1; BAM22; AP105A; CLAPB2
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
beta-Adaptin; Monoclonal Antibody; beta-Adaptin (N-terminal region) Antibody; beta-Adaptin (N-terminal region); Adaptin; anti-AP1B1 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M295
Specificity
This antibody detects a 106kDa* protein corresponding to the molecular mass of beta-Adaptin on SDS-PAGE immunoblots of human Jurkat cells.
Form/Format
Mouse monoclonal, protein G purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-AP1B1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:2000
WB: 1:1000
WB: 1:1000
Immunogen
Clone (M295) was generated from a sequence corresponding to amino acids near the N-terminal region of the human beta1 subunit of the adaptor related protein complex 1. This sequence has high homology to rat and mouse beta1-Adaptin.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-beta-Adaptin (N-terminal region) antibody at 1:500 (lane 1) and 1:2000 (lane 2).)
Western Blot (WB)
(Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-beta-Adaptin (N-terminal region) antibody at 1:500 (lane 1) and 1:2000 (lane 2).)
Related Product Information for anti-AP1B1 antibody
Adaptins are essential for the formation of adaptor complexes of clathrin-coded vesicles. Adaptins interact with the cytoplasmic domains of membrane-spanning receptors in the course of their endocytic/exocytic transport. They represent 1 of at least 4 components of intracellular transport and receptor downregulation pathways, the others being alpha/beta/gamma-adaptins, receptors, and ligand molecules. beta-adaptins have a bipartite structure with invariant N-terminal and variable C-terminal domains. The former interact with the uniform component of coded vesicles, the clathrin lattice. In contrast, the C-terminal domain provides a set of recognition sequences for other proteins and therefore functions as part of a selector domain responsible for the specific entrapment of membrane-bound proteins in vesicles.
References
Sosa, M.A. (1996) Biocell. 20(3):301.
Boehm M. & Bonifacino J.S. (2002) Gene. 286(2):175.
Boehm M. & Bonifacino J.S. (2002) Gene. 286(2):175.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101,348 Da
NCBI Official Full Name
AP-1 complex subunit beta-1 isoform a
NCBI Official Synonym Full Names
adaptor-related protein complex 1, beta 1 subunit
NCBI Official Symbol
AP1B1
NCBI Official Synonym Symbols
ADTB1; BAM22; AP105A; CLAPB2
NCBI Protein Information
AP-1 complex subunit beta-1; Golgi adaptor HA1/AP1 adaptin beta subunit; adapter-related protein complex 1 subunit beta-1; adaptor protein complex AP-1 subunit beta-1; adaptor-related protein complex 1 subunit beta-1; beta-1-adaptin; beta-adaptin 1; beta-
UniProt Protein Name
AP-1 complex subunit beta-1
Protein Family
UniProt Gene Name
AP1B1
UniProt Synonym Gene Names
ADTB1; BAM22; CLAPB2
UniProt Entry Name
AP1B1_HUMAN
Similar Products
Product Notes
The AP1B1 ap1b1 (Catalog #AAA474170) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta-Adaptin (N-terminal region) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Adaptin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:2000 WB: 1:1000. Researchers should empirically determine the suitability of the AP1B1 ap1b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Adaptin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
