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Flow Cytometry (FC/FACS)
(Figure 1. Detection of BCMA/ TNFRSF 17 in K562-BCMA (K562 cells transduced with gene for full length BCMA) Human Cell Line or NCI-H929 Human Cell Line with Rabbit Anti-Human BCMA/TNFRSF 17 Antigen Affinity-purified monoclonal antibody (clone: DM16) by Flow Cytometry.)
Rabbit anti-Human BCMA Monoclonal Antibody | anti-BCMA antibody
Anti-BCMA antibody (DM16), Rabbit mAb
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence
Purity
Purified from cell culture supernatant by affinity chromatography
Synonyms
BCMA; Monoclonal Antibody; Anti-BCMA antibody (DM16); Rabbit mAb; TNFRSF17; anti-BCMA antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
DM16
Purity/Purification
Purified from cell culture supernatant by affinity chromatography
Form/Format
Liquid; 0.1% Procline 300; 50% Glycerol; PBS, pH7.4; 0.1% BSA
Applicable Applications for anti-BCMA antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
FC:FACS: 1:100
IP 1:30
IP 1:30
Immunogen
Recombinant human BCMA (Met1-Ala54) produced by using human HEK293 cells
Reconstitution
Reconstitute with deionized water
Preservative
0.1% Procline 300
Constituents
50% Glycerol; PBS, pH 7.4; 0.1% BSA
Preparation and Storage
Store at -20 degree C for 12 months (Avoid repeated freezing and thawing)
Shipping: Blue Ice
Shipping: Blue Ice
Flow Cytometry (FC/FACS)
(Figure 1. Detection of BCMA/ TNFRSF 17 in K562-BCMA (K562 cells transduced with gene for full length BCMA) Human Cell Line or NCI-H929 Human Cell Line with Rabbit Anti-Human BCMA/TNFRSF 17 Antigen Affinity-purified monoclonal antibody (clone: DM16) by Flow Cytometry.)
Flow Cytometry (FC/FACS)
(Figure 1. Detection of BCMA/ TNFRSF 17 in K562-BCMA (K562 cells transduced with gene for full length BCMA) Human Cell Line or NCI-H929 Human Cell Line with Rabbit Anti-Human BCMA/TNFRSF 17 Antigen Affinity-purified monoclonal antibody (clone: DM16) by Flow Cytometry.)
Testing Data
(Figure 2. Binding of different clone Rabbit Anti-Human BCMA/TNFRSF 17 Antigen Affinity-purified monoclonal antibody to NCI-H929 and K562-BCMA cells was determined by flow cytometry.)
Testing Data
(Figure 2. Binding of different clone Rabbit Anti-Human BCMA/TNFRSF 17 Antigen Affinity-purified monoclonal antibody to NCI-H929 and K562-BCMA cells was determined by flow cytometry.)
Testing Data
(Figure 3. Phylogenetic analysis of different clone Rabbit Anti-Human BCMA/TNFRSF 17 Antigen Affinity-purified monoclonal antibody A) heavy chain and B) Light chain.)
Testing Data
(Figure 3. Phylogenetic analysis of different clone Rabbit Anti-Human BCMA/TNFRSF 17 Antigen Affinity-purified monoclonal antibody A) heavy chain and B) Light chain.)
ELISA (EIA)
(Figure 4. ELISA plate was coated with recombinant BCMA-hFc fusion protein, followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.)
ELISA (EIA)
(Figure 4. ELISA plate was coated with recombinant BCMA-hFc fusion protein, followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.)
Related Product Information for anti-BCMA antibody
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-BCMA antibody
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Product Notes
The BCMA (Catalog #AAA188040) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BCMA antibody (DM16), Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCMA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF). FC:FACS: 1:100 IP 1:30. Researchers should empirically determine the suitability of the BCMA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCMA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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