Mouse Bcl-2-like protein 1 Monoclonal Antibody | anti-Bcl-2 antibody

Mouse anti Human Bcl-2-like protein 1 monoclonal Antibody

Cat. Num. AAA1492459

• Gene Names: BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
• Reactivity: Human. Other species are not tested. Please decide the specificity by homology
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G Purified
• Synonyms: Bcl-2-like protein 1; Monoclonal Antibody; Mouse anti Human Bcl-2-like protein 1 monoclonal Antibody; Bcl2-L-1; Apoptosis regulator Bcl-X; BCL2L1; BCL2L; BCLX; anti-Bcl-2 antibody

• Host: Mouse
• Reactivity: Human. Other species are not tested. Please decide the specificity by homology
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Specific for Human Bcl-2-like protein 1 denatured and native forms
• Purity/Purification: >95%, Protein G Purified
• Sequence Length: 170

• Applicable Applications for anti-Bcl-2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant Human Bcl-2-like protein 1 protein
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-136207 / sc-8392 / sc-271121 / sc-23958 / sc-70417 / sc-70418 / sc-56021
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded Human endometrium tissue using MBS1492459 at dilution of 1:200)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded Human Colon cancer tissue using MBS1492459 at dilution of 1:200)

Western Blot (WB)

(All lanes: Mouse anti BCL2L1 Monoclonal antibody at 1ug/mlLane 1:HepG2 whole cell lysateSecondary Goat polyclonal to Mouse IgG at 1/5000 dilutionPredicted band size:26,27,19kdObserved band size:26KD)

Related Product Information for anti-Bcl-2 antibody

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.

NCBI and Uniprot Product Information

• NCBI GI #: 4502381
• NCBI GeneID: 598
• NCBI Accession #: NP_001182.1
• NCBI GenBank Nucleotide #: NM_001191.3
• UniProt Accession #: Q07817; Q5CZ89; Q5TE65; Q92976; E1P5L6
• Molecular Weight: 25,290 Da
• NCBI Official Full Name: bcl-2-like protein 1 isoform Bcl-X(S)
• NCBI Official Synonym Full Names: BCL2 like 1
• NCBI Official Symbol: BCL2L1
• NCBI Official Synonym Symbols: BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
• NCBI Protein Information: bcl-2-like protein 1
• UniProt Protein Name: Bcl-2-like protein 1
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L1
• UniProt Synonym Gene Names: BCL2L; BCLX; Bcl2-L-1
• UniProt Entry Name: B2CL1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]

Uniprot Description

Bcl-xL: an antiapoptotic member of the Bcl-2 family. Located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are potent inducers of cell apoptosis. Two alternatively spliced isoforms have been reported.

Protein type: Autophagy; Mitochondrial; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: cell junction; centrosome; cytoplasm; cytosol; integral to membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrial outer membrane; mitochondrion; nuclear membrane; synaptic vesicle membrane

Molecular Function: BH3 domain binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding

Biological Process: apoptosis; apoptotic mitochondrial changes; cell proliferation; cellular process regulating host cell cycle in response to virus; cytokinesis; DNA damage response, signal transduction resulting in induction of apoptosis; endocytosis; fertilization; germ cell development; growth; in utero embryonic development; innate immune response; male gonad development; mitotic cell cycle checkpoint; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of neuron apoptosis; neuron apoptosis; ovarian follicle development; positive regulation of cell proliferation; programmed cell death; regulation of mitochondrial membrane permeability; regulation of mitochondrial membrane potential; release of cytochrome c from mitochondria; response to cycloheximide; response to cytokine stimulus; spermatogenesis; suppression by virus of host apoptosis