Mouse Bcl-2 Monoclonal Antibody
Mouse Anti-Bcl-2-UNLB
Applications
ELISA, Western Blot
Synonyms
Bcl-2; Monoclonal Antibody; Mouse Anti-Bcl-2-UNLB; Mouse Anti-Bcl-2 - Purified; Unlabeled Antibody; Mouse Anti-Mouse/Rat Bcl-2; anti-Bcl-2 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
10C2
Specificity
Mouse and rat Bcl-2 (Mr 26 kDa)
Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems.2 It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment.3 Bcl-2 is a widely studied protein that has been shown to be a potent inhibitor of programmed cell death. It has been localized to the outer mitochondrial membrane, perinuclear membrane, and endoplasmic reticulum. Bcl-2 is expressed in memory and resting, or other long-lived lymphoid cells, follicular mantle cells, medullary thymocytes, and lymphomas. Germical center cells and cortical thymocytes are negative for Bcl-2. Upregulation of Bcl-2 prevents or delays apoptosis induced by a variety of stimuli, including growth factor deprivation, gamma -irradiation, glucocorticoids, and chemotherapeutic agents. During lymphoid development, expression of the Bcl-2 protein appears to be regulated in a stage-specific manner, and is thought to be a survival signal for positive selection. The monoclonal antibody 10C2 reacts with both mouse and rat Bcl-2.1
Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems.2 It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment.3 Bcl-2 is a widely studied protein that has been shown to be a potent inhibitor of programmed cell death. It has been localized to the outer mitochondrial membrane, perinuclear membrane, and endoplasmic reticulum. Bcl-2 is expressed in memory and resting, or other long-lived lymphoid cells, follicular mantle cells, medullary thymocytes, and lymphomas. Germical center cells and cortical thymocytes are negative for Bcl-2. Upregulation of Bcl-2 prevents or delays apoptosis induced by a variety of stimuli, including growth factor deprivation, gamma -irradiation, glucocorticoids, and chemotherapeutic agents. During lymphoid development, expression of the Bcl-2 protein appears to be regulated in a stage-specific manner, and is thought to be a survival signal for positive selection. The monoclonal antibody 10C2 reacts with both mouse and rat Bcl-2.1
Form/Format
Purified (UNLB) Antibody
Sequence Length
154
Applicable Applications for anti-Bcl-2 antibody
ELISA; Western Blot
Application Notes
Western Blot Suggested Dilution: <= 1 ug/ml
Immunogen
KLH-conjugated peptide corresponding to 61-76 amino acid sequence of murine Bcl-21
Characterization
To insure lot-to-lot consistency, each batch of product is tested by immunoassay to conform to the characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The products should be stored at 2-8 degree C. Each product is stable for the period shown on the label if stored as directed.
The products should be stored at 2-8 degree C. Each product is stable for the period shown on the label if stored as directed.
References
2. Cohen, J.J. 1991. Adv. Immunol. 50:55.
3. Cohen, J.J., and R.C. Duke. 1992. Annu. Rev. Immunol. 10:2676.
4. Nunez, G., and M.F. Clarke. 1994. Trends Cell Biol. 4:399.
5. Cory, S. 1995. Annu. Rev. Immunol. 13:513.
6. Oltvai., Z.N., C.L. Millman, and S.J. Korsmeyer. 1993. Cell 74:609.
7. Farrow, S.N., et al. 1995. Nature 374:731.
8. Chittenden, T., et al. 1995. Nature 374:733.
9. Kiefer, M.C., et al. 1995. Nature 374:736.
10. Shimizu, S., et al. 1995. Nature 374:811.
11. Yin, X.M., Z.N. Oltvai, and S.J. Korsmeyer. 1994. Nature 369:321.
12. Cheng, E.H.-Y., et al. 1996. Nature 379:554.
13. Gottschalk, A.R., L.H. Boise, Z.N. Oltvai, M.A. Accavitti, S.J. Korsmeyer, J. Quintans, and C.B. Thompson. 1996. Cell Death and Differentiation 3:113.
3. Cohen, J.J., and R.C. Duke. 1992. Annu. Rev. Immunol. 10:2676.
4. Nunez, G., and M.F. Clarke. 1994. Trends Cell Biol. 4:399.
5. Cory, S. 1995. Annu. Rev. Immunol. 13:513.
6. Oltvai., Z.N., C.L. Millman, and S.J. Korsmeyer. 1993. Cell 74:609.
7. Farrow, S.N., et al. 1995. Nature 374:731.
8. Chittenden, T., et al. 1995. Nature 374:733.
9. Kiefer, M.C., et al. 1995. Nature 374:736.
10. Shimizu, S., et al. 1995. Nature 374:811.
11. Yin, X.M., Z.N. Oltvai, and S.J. Korsmeyer. 1994. Nature 369:321.
12. Cheng, E.H.-Y., et al. 1996. Nature 379:554.
13. Gottschalk, A.R., L.H. Boise, Z.N. Oltvai, M.A. Accavitti, S.J. Korsmeyer, J. Quintans, and C.B. Thompson. 1996. Cell Death and Differentiation 3:113.
NCBI and Uniprot Product Information
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Product Notes
The Bcl-2 (Catalog #AAA670009) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Bcl-2 can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot. Western Blot Suggested Dilution: <= 1 ug/ml. Researchers should empirically determine the suitability of the Bcl-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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