Mouse anti-Human B2M / Beta 2 Microglobulin Monoclonal Antibody | anti-B2M antibody

Mouse Monoclonal (IgG2a) to Human B2M / Beta 2 Microglobulin

Cat. Num. AAA245255

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, ELISA
• Purity: Ion exchange Chromatography
• Synonyms: B2M / Beta 2 Microglobulin; Monoclonal Antibody; Mouse Monoclonal (IgG2a) to Human B2M / Beta 2 Microglobulin; Anti-B2M / Beta 2 Microglobulin Antibody IHC-plus; B2M; Beta 2 Microglobulin; Beta-2-microglobulin; Beta-2-microglobin; Human B2M; anti-B2M antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: Recognizes human b2-microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as soluble human b2-microglobulin. Does not cross-react with mouse, bovine, rabbit, dog or chicken b2-microglobulin.
• Purity/Purification: Ion exchange Chromatography
• Form/Format: PBS, pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 119

• Applicable Applications for anti-B2M antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: ELISA, Flo (1 ug/ml), IHC-P (10 ug/ml), IP, WB (2 - 4 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Purified human b2-microglobulin.
• Immunogen Type: Purified protein
• Immunogen: B2M / Beta 2 Microglobulin antibody was raised against purified human b2-microglobulin.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-B2M antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-B2M antibody

Beta 2-microglobulin is the low molecular weight component of HLA antigens and as such is involved in the presentation of endogenous antigens, activation of T lymphocytes and functional emergence of natural killer cells. It is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning and other conditions leading renal tubular dysfunction.

NCBI and Uniprot Product Information

• NCBI GI #: 4757826
• NCBI GeneID: 567
• NCBI Accession #: NP_004039.1
• NCBI GenBank Nucleotide #: NM_004048.2
• UniProt Accession #: P61769; P01884; Q540F8; Q6IAT8; Q9UCK0; Q9UD48; Q9UDF4
• Molecular Weight: 13,715 Da
• NCBI Official Full Name: beta-2-microglobulin
• NCBI Official Synonym Full Names: beta-2-microglobulin
• NCBI Official Symbol: B2M
• NCBI Protein Information: beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
• UniProt Protein Name: Beta-2-microglobulin
• Protein Family: Beta-2-microglobulin
• UniProt Gene Name: B2M
• UniProt Entry Name: B2MG_HUMAN

NCBI Description

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Uniprot Description

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; endoplasmic reticulum lumen; early endosome membrane; cytoplasm; extracellular region; plasma membrane; MHC class I protein complex; external side of plasma membrane

Molecular Function: identical protein binding; protein binding

Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; antigen processing and presentation of peptide antigen via MHC class I; iron ion homeostasis; response to cadmium ion; response to molecule of bacterial origin; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent

Disease: Hypoproteinemia, Hypercatabolic