Mouse ATPase, Ca Monoclonal Antibody
ATPase, Ca (Plasma Membrane, PMCA)
Reactivity
Canine, Chicken, Feline, Human, Monkey, Mouse, Rabbit, Rat, Sheep
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Purity
AscitesAscites
Synonyms
ATPase; Ca; Monoclonal Antibody; Ca (Plasma Membrane; PMCA); Anti -ATPase; anti-ATPase; Ca antibody
Host
Mouse
Reactivity
Canine, Chicken, Feline, Human, Monkey, Mouse, Rabbit, Rat, Sheep
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6D131
Specificity
Recognizes all known (currently four) isoforms of human plasma membrane PMCA ATPase. Recognizes an epitope between aa724-783 of human APTase, Ca. Species Crossreactivity: rat, mouse, sheep, canine, monkey, rabbit, feline, chicken, amphibian
Purity/Purification
Ascites
Ascites
Ascites
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide.
Applicable Applications for anti-ATPase, Ca antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot.
Dilution: Immunohistochemistry (Frozen, paraffin): 1:500
Western Blot: 1:1000. Detects a band of ~140kD. Also detects bands at ~95kD and ~180kD which may represent aggregation and/or natural proteolytic products of PMCA ATPase.
Optimal dilution to be determined by the researcher.
Dilution: Immunohistochemistry (Frozen, paraffin): 1:500
Western Blot: 1:1000. Detects a band of ~140kD. Also detects bands at ~95kD and ~180kD which may represent aggregation and/or natural proteolytic products of PMCA ATPase.
Optimal dilution to be determined by the researcher.
Immunogen
Purified human erythrocyte PMCA ATPase (plasma membrane calcium pump ATPase).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-ATPase, Ca antibody
Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuous removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX) in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+ and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2).
Product Categories/Family for anti-ATPase, Ca antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ATPase
Similar Products
Product Notes
The ATPase, Ca (Catalog #AAA632097) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATPase, Ca (Plasma Membrane, PMCA) reacts with Canine, Chicken, Feline, Human, Monkey, Mouse, Rabbit, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's ATPase, Ca can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot. Dilution: Immunohistochemistry (Frozen, paraffin): 1:500 Western Blot: 1:1000. Detects a band of ~140kD. Also detects bands at ~95kD and ~180kD which may represent aggregation and/or natural proteolytic products of PMCA ATPase. Optimal dilution to be determined by the researcher. Researchers should empirically determine the suitability of the ATPase, Ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPase, Ca, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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