Mouse anti-Mouse ATG4C Monoclonal Antibody | anti-ATG4C antibody

ATG4C (ATG4C, APG4C, AUTL1, AUTL3, Cysteine protease ATG4C, AUT-like 3 cysteine endopeptidase, Autophagin-3, Autophagy-related cysteine endopeptidase 3, Autophagy-related protein 4 homolog C) (Azide free) (HRP)

Cat. Num. AAA6121458

• Gene Names: ATG4C; APG4C; AUTL1; AUTL3; APG4-C
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: ATG4C; Monoclonal Antibody; ATG4C (ATG4C; APG4C; AUTL1; AUTL3; Cysteine protease ATG4C; AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C) (Azide free) (HRP); Autophagy-related protein 4 homolog C; anti-ATG4C antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 287CT12.2.2
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-ATG4C antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1000; WB: 1:100; Applications are based on unconjugated antibody.
• Immunogen: ATG4C Mab is generated from mouse immunized with ATG4C recombinant protein.
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ATG4C Antibody western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ATG4C antibody detected the ATG4C protein (arrow).)

Related Product Information for anti-ATG4C antibody

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq].

Product Categories/Family for anti-ATG4C antibody

Antibodies; Autophagy

References

Kathiresan, S., et al. Nat. Genet. 40(2):189-197(2008) Marino, G., et al. J. Biol. Chem. 282(25):18573-18583(2007) Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007) Marino, G., et al. J. Biol. Chem. 278(6):3671-3678(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 30410844
• NCBI GeneID: 84938
• NCBI Accession #: NP_116241.2; NP_835739.1
• NCBI GenBank Nucleotide #: NM_032852.3
• UniProt Accession #: Q96DT6; Q96K04; A6NLR8; D3DQ58
• Molecular Weight: 52,497 Da
• NCBI Official Full Name: cysteine protease ATG4C
• NCBI Official Synonym Full Names: autophagy related 4C, cysteine peptidase
• NCBI Official Symbol: ATG4C
• NCBI Official Synonym Symbols: APG4C; AUTL1; AUTL3; APG4-C
• NCBI Protein Information: cysteine protease ATG4C; autophagin-3; APG4 autophagy 4 homolog C; AUT-like 3 cysteine endopeptidase; ATG4 autophagy related 4 homolog C; AUT-like 1, cysteine endopeptidase; autophagy-related protein 4 homolog C; autophagy-related cysteine endopeptidase 3
• UniProt Protein Name: Cysteine protease ATG4C
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4C
• UniProt Synonym Gene Names: APG4C; AUTL1; AUTL3
• UniProt Entry Name: ATG4C_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4C: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Highly expressed in skeletal muscle, heart, liver and testis. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family.

Protein type: EC 3.4.22.-; Protease; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: cytoplasm; extracellular region; cytosol

Molecular Function: peptidase activity; cysteine-type endopeptidase activity

Biological Process: protein delipidation; mitochondrion degradation; autophagy; protein processing; C-terminal protein lipidation; proteolysis; protein targeting to membrane; autophagic vacuole formation; cellular response to nitrogen starvation