Mouse anti-Human ATF1 Monoclonal Antibody | anti-ATF1 antibody

ATF1 (Cyclic AMP-dependent Transcription Factor ATF-1, cAMP-dependent Transcription Factor ATF-1, Activating Transcription Factor 1, Protein TREB36) (Biotin)

Cat. Num. AAA6140714

• Gene Names: ATF1; TREB36; EWS-ATF1; FUS/ATF-1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATF1; Monoclonal Antibody; ATF1 (Cyclic AMP-dependent Transcription Factor ATF-1; cAMP-dependent Transcription Factor ATF-1; Activating Transcription Factor 1; Protein TREB36) (Biotin); anti-ATF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3E7
• Specificity: Recognizes human ATF1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ATF1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-271 from human ATF1 (AAH29619) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (55.55kD).)

Western Blot (WB)

(Western Blot analysis of ATF1 expression in transfected 293T cell line by ATF1 monoclonal antibody. Lane 1: ATF1 transfected lysate (29.2kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to ATF1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged ATF1 is 0.3ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of ATF1 over-expressed 293 cell line, cotransfected with ATF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ATF1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Product Categories/Family for anti-ATF1 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 20810444
• NCBI GeneID: 466
• Molecular Weight: 15,295 Da
• NCBI Official Full Name: Homo sapiens activating transcription factor 1, mRNA
• NCBI Official Synonym Full Names: activating transcription factor 1
• NCBI Official Symbol: ATF1
• NCBI Official Synonym Symbols: TREB36; EWS-ATF1; FUS/ATF-1
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-1
• Protein Family: Alcohol O-acetyltransferase

NCBI Description

This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]