Mouse ASH1L1 Monoclonal Antibody | anti-ASH1L antibody

ASH1L1 (Ash1 (absent, small, or homeotic)-like, ASH1L, FLJ10504, KIAA1420)

Cat. Num. AAA601680

• Gene Names: ASH1L; ASH1; KMT2H; ASH1L1; FLJ10504; KIAA1420
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: ASH1L1; Monoclonal Antibody; ASH1L1 (Ash1 (absent; small; or homeotic)-like; ASH1L; FLJ10504; KIAA1420); Anti -ASH1L1 (Ash1 (absent; anti-ASH1L antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C18
• Specificity: Recognizes human ASH1L1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-ASH1L antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Partial sequence of recombinant full-length protein to human ASH1L1
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-ASH1L antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 225000936
• NCBI GeneID: 55870
• UniProt Accession #: Q9NR48; Q59GP1; Q5T714; Q5T715; Q9P2C7
• Molecular Weight: 332,790 Da
• NCBI Official Full Name: Ash1 (absent, small, or homeotic)-like (Drosophila)
• NCBI Official Synonym Full Names: ash1 (absent, small, or homeotic)-like (Drosophila)
• NCBI Official Symbol: ASH1L
• NCBI Official Synonym Symbols: ASH1; KMT2H; ASH1L1; FLJ10504; KIAA1420
• NCBI Protein Information: probable histone-lysine N-methyltransferase ASH1L; ASH1-like protein; OTTHUMP00000015897; OTTHUMP00000015898; lysine N-methyltransferase 2H; absent small and homeotic disks protein 1 homolog
• UniProt Protein Name: Probable histone-lysine N-methyltransferase ASH1L
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: ASH1L
• UniProt Synonym Gene Names: KIAA1420; KMT2H
• UniProt Entry Name: ASH1L_HUMAN

NCBI Description

This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. [provided by RefSeq]

Uniprot Description

ASH1L: a protein methyltransferase that specifically methylates K36 of histone H3 (H3K36me). A member of the trithorax group (trxG) of transcriptional activators that is required for proper expression of Hox genes and is preferentially expressed in haematopoietic stem cells. Widely expressed, with high levels in brain, heart and kidney. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions.

Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine; Cell adhesion; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; Golgi apparatus; tight junction; cytoplasm; chromosome; nucleus

Molecular Function: DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); chromatin binding

Biological Process: transcription from RNA polymerase II promoter; cell-cell signaling; interleukin-6 production; negative regulation of MAPKKK cascade; histone H3-K4 methylation; negative regulation of acute inflammatory response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of I-kappaB kinase/NF-kappaB cascade; DNA packaging