Mouse anti-Human ARID3A / DRIL1 Monoclonal Antibody | anti-ARID3A antibody

Mouse Monoclonal [clone 1A11] (IgG1,k) to Human ARID3A / DRIL1

Cat. Num. AAA246633

• Gene Names: ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: ARID3A / DRIL1; Monoclonal Antibody; Mouse Monoclonal [clone 1A11] (IgG1; k) to Human ARID3A / DRIL1; Anti-ARID3A / DRIL1 Antibody (clone 1A11) IHC-plus; ARID3A; ARID domain-containing 3A; BRIGHT; Dead ringer-like protein 1; Dead ringer-like 1; DRX; E2FBP1; E2F-binding protein 1; DRIL1; DRIL3; Human ARID3A; anti-ARID3A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1A11
• Specificity: Human ARID3A
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 593

• Applicable Applications for anti-ARID3A antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: ARID3A (NP_005215, 317 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: ARID3A / DRIL1 antibody was raised against aRID3A (NP_005215, 317 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-ARID3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(ARID3A monoclonal antibody clone 1A11 Western blot of ARID3A expression in K-562.)

ELISA (EIA)

(Detection limit for recombinant GST tagged ARID3A is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4885193
• NCBI GeneID: 1820
• NCBI Accession #: NP_005215.1
• NCBI GenBank Nucleotide #: NM_005224.2
• UniProt Accession #: Q99856; Q5I858; Q6P9C6; Q8IZA7; Q8N4Z3
• Molecular Weight: 62,889 Da
• NCBI Official Full Name: AT-rich interactive domain-containing protein 3A
• NCBI Official Synonym Full Names: AT rich interactive domain 3A (BRIGHT-like)
• NCBI Official Symbol: ARID3A
• NCBI Official Synonym Symbols: DRIL1; DRIL3; BRIGHT; E2FBP1
• NCBI Protein Information: AT-rich interactive domain-containing protein 3A; dead ringer-like 1; E2F-binding protein 1; ARID domain-containing 3A; dead ringer-like protein 1; ARID domain-containing protein 3A; B-cell regulator of IgH transcription; AT rich interactive domain 3A (BRIGHT- like) protein
• UniProt Protein Name: AT-rich interactive domain-containing protein 3A
• Protein Family: AT-rich interactive domain-containing protein
• UniProt Gene Name: ARID3A
• UniProt Synonym Gene Names: DRIL1; DRIL3; DRX; E2FBP1; ARID domain-containing protein 3A; Bright
• UniProt Entry Name: ARI3A_HUMAN

NCBI Description

This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification. [provided by RefSeq, Jul 2008]

Uniprot Description

Bright: a member of the ARID (AT-rich interaction domain) family of transcription factors. It was found by its homology to the Drosophila dead ringer protein, which is important for normal embryogenesis. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. Contributes to localized control of accessibility and therefore nonrandom gene use during V(D)J recombination.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; lipid raft

Molecular Function: protein binding; protein homodimerization activity; chromatin binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter