Western Blot (WB)
(Western Blot detection against Immunogen (83.2kD).)
Mouse anti-Human ARHGEF5 Monoclonal Antibody | anti-ARHGEF5 antibody
ARHGEF5 (TIM, Rho Guanine Nucleotide Exchange Factor 5, Ephexin-3, Guanine Nucleotide Regulatory Protein TIM, Oncogene TIM, Transforming Immortalized Mammary Oncogene, p60 TIM, DKFZp686N1969, GEF5, P60, TIM1) (Biotin)
Gene Names
ARHGEF5; P60; TIM; GEF5; TIM1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ARHGEF5; Monoclonal Antibody; ARHGEF5 (TIM; Rho Guanine Nucleotide Exchange Factor 5; Ephexin-3; Guanine Nucleotide Regulatory Protein TIM; Oncogene TIM; Transforming Immortalized Mammary Oncogene; p60 TIM; DKFZp686N1969; GEF5; P60; TIM1) (Biotin); anti-ARHGEF5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3A12-B5
Specificity
Recognizes human ARHGEF5.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Sequence Length
2450
Applicable Applications for anti-ARHGEF5 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-520 from ARHGEF5 (AAH14555) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQP
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (83.2kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (83.2kD).)
Western Blot (WB)
(Western Blot analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody Lane 1: ARHGEF5 transfected lysate (60.1kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody Lane 1: ARHGEF5 transfected lysate (60.1kD). Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged ARHGEF5 is 1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged ARHGEF5 is 1ng/ml as a capture antibody.)
Related Product Information for anti-ARHGEF5 antibody
Mainly expressed in kidney, liver, pancreas, lung and placenta.
Product Categories/Family for anti-ARHGEF5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens Rho guanine nucleotide exchange factor (GEF) 5, mRNA
NCBI Official Synonym Full Names
Rho guanine nucleotide exchange factor 5
NCBI Official Symbol
ARHGEF5
NCBI Official Synonym Symbols
P60; TIM; GEF5; TIM1
NCBI Protein Information
rho guanine nucleotide exchange factor 5
Protein Family
NCBI Description
Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008]
Research Articles on ARHGEF5
Similar Products
Product Notes
The ARHGEF5 (Catalog #AAA6140659) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ARHGEF5 (TIM, Rho Guanine Nucleotide Exchange Factor 5, Ephexin-3, Guanine Nucleotide Regulatory Protein TIM, Oncogene TIM, Transforming Immortalized Mammary Oncogene, p60 TIM, DKFZp686N1969, GEF5, P60, TIM1) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGEF5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ARHGEF5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGEF5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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