Mouse anti-Human ANXA3 Monoclonal Antibody | anti-ANXA3 antibody

ANXA3 (Annexin A3, Annexin-3, Annexin III, Lipocortin III, Placental Anticoagulant Protein III, PAP-III, 35-alpha Calcimedin, Inositol 1,2-cyclic Phosphate 2-phosphohydrolase, ANX3) (HRP)

Cat. Num. AAA6151218

• Gene Names: ANXA3; ANX3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ANXA3; Monoclonal Antibody; ANXA3 (Annexin A3; Annexin-3; Annexin III; Lipocortin III; Placental Anticoagulant Protein III; PAP-III; 35-alpha Calcimedin; Inositol 1; 2-cyclic Phosphate 2-phosphohydrolase; ANX3) (HRP); anti-ANXA3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4F1
• Specificity: Recognizes human ANXA3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-ANXA3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-323 from human ANXA3 (AAH00871) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGDD
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (61.27kD).)

Western Blot (WB)

(Western Blot analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody. Lane 1: ANXA3 transfected lysate (37.145kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged ANXA3 is ~1ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of ANXA3 over-expressed 293 cell line, cotransfected with ANXA3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ANXA3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Related Product Information for anti-ANXA3 antibody

ANXA3 is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.

Product Categories/Family for anti-ANXA3 antibody

Antibodies; Binding Proteins

References

1. A multiplex model of combining gene-based, protein-based, and metabolite-based with positive and negative markers in urine for the early diagnosis of prostate cancer. Cao DL, Ye DW, Zhang HL, Zhu Y, Wang YX, Yao XD.Prostate. 2010 Oct 18.

NCBI and Uniprot Product Information

• NCBI GI #: 12654114
• NCBI GeneID: 306
• Molecular Weight: 36,375 Da
• NCBI Official Full Name: Homo sapiens annexin A3, mRNA
• NCBI Official Synonym Full Names: annexin A3
• NCBI Official Symbol: ANXA3
• NCBI Official Synonym Symbols: ANX3
• NCBI Protein Information: annexin A3
• Protein Family: Annexin

NCBI Description

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq, Jul 2008]