Mouse ANK3 / ANKYRIN-G Monoclonal Antibody | anti-ANK3 antibody

Mouse Monoclonal [clone S106-20] (IgG1)

Cat. Num. AAA245524

• Gene Names: ANK3; MRT37; ANKYRIN-G
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: ANK3 / ANKYRIN-G; Monoclonal Antibody; Mouse Monoclonal [clone S106-20] (IgG1); Anti-ANK3 / ANKYRIN-G Antibody (clone S106-20) IHC-plus; ANK3; ANKYRIN-G; ANK-3; Ankyrin G119; Ankyrin-3; anti-ANK3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S106-20
• Specificity: Detects over 200kDa.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH7.4, 50% glycerol and 0.09% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 4377

• Applicable Applications for anti-ANK3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (20 ug/ml), WB
• Immunogen Description: Fusion protein 1000 C-terminal amino acids of human Ankyrin G encompassing all of Ankyrin G with the exception of ankyrin repeats.
• Immunogen Type: Fusion protein
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-ANK3 / ANKYRIN-G antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ANK3 / ANKYRIN-G antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of Ankyrin-G in rat brain membrane tissues, using a 1:1000 dilution of ANK3 / ANKYRIN-G antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 32967601
• NCBI GeneID: 288
• NCBI Accession #: NP_066267.2
• NCBI GenBank Nucleotide #: NM_020987.3
• UniProt Accession #: Q12955; Q13484; Q5CZH9; Q5VXD5; Q7Z3G4; Q9H0P5; B1AQT2; B4DIL1; E9PE32
• Molecular Weight: 160,947 Da
• NCBI Official Full Name: ankyrin-3 isoform 1
• NCBI Official Synonym Full Names: ankyrin 3, node of Ranvier (ankyrin G)
• NCBI Official Symbol: ANK3
• NCBI Official Synonym Symbols: MRT37; ANKYRIN-G
• NCBI Protein Information: ankyrin-3; ankyrin G119
• UniProt Protein Name: Ankyrin-3
• Protein Family: Ankyrin
• UniProt Gene Name: ANK3
• UniProt Synonym Gene Names: ANK-3
• UniProt Entry Name: ANK3_HUMAN

NCBI Description

Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

ANK3: In skeletal muscle, required for costamere localization of DMD and betaDAG1. Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Genetic variations in ANK3 are associated with autism spectrum disorders susceptibility. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: Golgi apparatus; cell surface; tight junction; costamere; sarcoplasmic reticulum; basolateral plasma membrane; lysosome; endoplasmic reticulum; paranode region of axon; dendrite; T-tubule; Z disc; postsynaptic membrane; plasma membrane; basal plasma membrane; neuromuscular junction; lateral plasma membrane; sarcolemma

Molecular Function: protein binding, bridging; protein binding; spectrin binding; structural constituent of cytoskeleton; cadherin binding; cytoskeletal protein binding

Biological Process: axon guidance; cytoskeletal anchoring; regulation of potassium ion transport; generation of action potential; signal transduction; positive regulation of homotypic cell-cell adhesion; establishment of protein localization; cytokinesis after mitosis; axonogenesis; plasma membrane organization and biogenesis; Golgi to plasma membrane protein transport; positive regulation of membrane potential; neuromuscular junction development

Disease: Mental Retardation, Autosomal Recessive 37