Mouse Amyloid Beta 1-42 Monoclonal Antibody | anti-Abeta42 antibody
Amyloid Beta 1-42 (Abeta42) Monoclonal Antibody
Applications
Immunoblot, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Synonyms
Amyloid Beta 1-42; Monoclonal Antibody; Amyloid Beta 1-42 (Abeta42) Monoclonal Antibody; Anti-Amyloid Beta 1-42; anti-Abeta42 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
This antibody specifically recognizes an epitope within Abetax-42. NOTE: When administered to young Tg2576 mice with minimal Abeta deposition, this antibody reduced Abeta accumulation in the brain.
Form/Format
100ug in PBS, pH 7.4. Purified by Protein G affinity chromatography.
Applicable Applications for anti-Abeta42 antibody
Immunoblot (IB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
mmunoblotting, Immunohistochemistry: Im m unofluorescence, Immunoprecip i tation Test at 1-10ug/ml. Sandwich ELISA (as capture antibody with #MBS190935 as HRP - c onjugated detection antibody). These are recommended concentrations; enduser should determine optimal concentrations for their applications. Sandwich ELISA protoco l on next page. See specific product references below for more information.
Sandwich ELISA Protocol:
96-well ELISA plates are coated with capture MAbat 2.5-5ug/well and incubated at 4 degree C overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4 degree C overnight. The next day plates are washed in PBS and serial dilution of A beta samples are added; plates are incubated overnight at 4 degree C. The next day plates are washed in PBS, and HRP-conjugated detection Abeta MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins
Sandwich ELISA Protocol:
96-well ELISA plates are coated with capture MAbat 2.5-5ug/well and incubated at 4 degree C overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4 degree C overnight. The next day plates are washed in PBS and serial dilution of A beta samples are added; plates are incubated overnight at 4 degree C. The next day plates are washed in PBS, and HRP-conjugated detection Abeta MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins
Antigen
Synthetic peptide corresponding to Abeta35-42.
Dilution Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C.
Related Product Information for anti-Abeta42 antibody
Accumulation and aggregation of amyloid beta (Abeta) in the brain is indicated as the trigger of a pathological cascade that causes Alzheimer disease (AD). The highly amyloidogenic 42-amino acid form of Abeta (Abeta42) and aminoterminally truncated forms of Abeta (Abetax-42) are the predominant species of Abeta typically found in diffuse and senile plaques within the AD brain. The relative levels of Abeta42 appear to be the key regulators of Abeta aggregation into amyloid; thus Abeta42 has been implicated as the initiating molecule in the pathogenesis of AD.
Product Categories/Family for anti-Abeta42 antibody
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Product Notes
The Abeta42 (Catalog #AAA190932) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Amyloid Beta 1-42 can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). mmunoblotting, Immunohistochemistry: Im m unofluorescence, Immunoprecip i tation Test at 1-10ug/ml. Sandwich ELISA (as capture antibody with #MBS190935 as HRP - c onjugated detection antibody). These are recommended concentrations; enduser should determine optimal concentrations for their applications. Sandwich ELISA protoco l on next page. See specific product references below for more information. Sandwich ELISA Protocol: 96-well ELISA plates are coated with capture MAbat 2.5-5ug/well and incubated at 4 degree C overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4 degree C overnight. The next day plates are washed in PBS and serial dilution of A beta samples are added; plates are incubated overnight at 4 degree C. The next day plates are washed in PBS, and HRP-conjugated detection Abeta MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins. Researchers should empirically determine the suitability of the Abeta42 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Amyloid Beta 1-42, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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