Mouse AMPK alpha 1 Monoclonal Antibody | anti-PRKAA1 antibody

Anti-AMPK alpha 1 Antibody

Cat. Num. AAA8241475

• Gene Names: PRKAA1; AMPK; AMPKa1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Synonyms: AMPK alpha 1; Monoclonal Antibody; Anti-AMPK alpha 1 Antibody; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; anti-PRKAA1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Specificity: Recognizes endogenous levels of AMPK alpha 1 protein.
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
• Sequence Length: 559

• Applicable Applications for anti-PRKAA1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blot: (1/1000 - 1/2000); Immunohistochemistry: (1/100 - 1/200)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human AMPK alpha 1. The exact sequence is proprietary.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of AMPK alpha 1 expression in Hela (A), 293T (B), NIH3T3 (C), PC12 (D) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of AMPK alpha 1 staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Related Product Information for anti-PRKAA1 antibody

Mouse monoclonal to AMPK alpha 1

NCBI and Uniprot Product Information

• NCBI GI #: 254763436
• NCBI GeneID: 5562
• UniProt Accession #: Q13131; O00286; Q5D0E1; Q86VS1; Q9UNQ4; A8MTQ6; B2R7E1
• Molecular Weight: 65,523 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• NCBI Official Synonym Full Names: protein kinase AMP-activated catalytic subunit alpha 1
• NCBI Official Symbol: PRKAA1
• NCBI Official Synonym Symbols: AMPK; AMPKa1
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Gene Name: PRKAA1
• UniProt Synonym Gene Names: AMPK1; HMGCR kinase
• UniProt Entry Name: AAPK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

AMPKA1: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.26; EC 2.7.11.31; Protein kinase, Ser/Thr (non-receptor); Autophagy; Protein kinase, CAMK; EC 2.7.11.27; EC 2.7.11.1; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: AMP-activated protein kinase complex; apical plasma membrane; cytoplasm; cytosol; intracellular; nucleus

Molecular Function: [acetyl-CoA carboxylase] kinase activity; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase activity; chromatin binding; histone serine kinase activity; kinase binding; metal ion binding; protein binding; protein C-terminus binding; protein kinase activity; tau-protein kinase activity

Biological Process: activation of MAPK activity; cell cycle arrest; cellular response to glucose starvation; cellular response to nutrient levels; cholesterol biosynthetic process; cold acclimation; fatty acid biosynthetic process; fatty acid homeostasis; fatty acid oxidation; gene expression; glucose homeostasis; glucose metabolic process; insulin receptor signaling pathway; lipid biosynthetic process; macroautophagy; negative regulation of apoptosis; negative regulation of glucosylceramide biosynthetic process; negative regulation of lipid catabolic process; negative regulation of TOR signaling pathway; positive regulation of autophagy; positive regulation of cell proliferation; positive regulation of cholesterol biosynthetic process; positive regulation of glycolysis; positive regulation of skeletal muscle development; protein amino acid phosphorylation; protein heterooligomerization; regulation of circadian rhythm; regulation of peptidyl-serine phosphorylation; regulation of transcription, DNA-dependent; response to activity; response to caffeine; response to gamma radiation; response to hypoxia; response to UV; rhythmic process; signal transduction; transcription initiation from RNA polymerase II promoter; Wnt receptor signaling pathway