Rat anti-Human Aminoacylase 1 Monoclonal Antibody | anti-ACY1 antibody

Aminoacylase 1 (ACY1)

Cat. Num. AAA606305

• Gene Names: ACY1; ACY-1; ACY1D
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Aminoacylase 1; Monoclonal Antibody; Aminoacylase 1 (ACY1); Anti -Aminoacylase 1 (ACY1); anti-ACY1 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone Number: 9L801
• Specificity: Recognizes human Aminocyclase. Species sequence homology: chimpanzee (100%), canine (88%), mouse and rat (86%).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

• Applicable Applications for anti-ACY1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunoprecipitation.; Dilution: ELISA: 0.5-1ug/ml; Immunoprecipitation: 25ug/ml; Western Blot: 1-2ug/ml
• Immunogen: Purified, insect cell-derived, recombinant human aminoacylase (aa2-408).
• Hybridoma: Mouse myeloma cells with B cells obtained from a rat immunized with purified, insect cell-derived, recombinant human aminoacylase (aa2-408).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ACY1 antibody

Aminoacylase (ACY1) is an intracellular soluble homodimeric zinc-binding enzyme. It is involved in the breakdown of acetylated amino acids generated during protein degradation by catalyzing the formation of free aliphatic amino acids from N-acetylated precursors. Mutations in the ACY1 gene might cause inborn metabolic disorders. The aa sequence of human ACY1 is 100%, 88% and 86% identical to that of chimpanzee, dog and mouse/rat.

Product Categories/Family for anti-ACY1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 12804329
• NCBI GeneID: 95
• UniProt Accession #: Q03154
• Molecular Weight: 45,885 Da
• NCBI Official Full Name: Aminoacylase 1
• NCBI Official Synonym Full Names: aminoacylase 1
• NCBI Official Symbol: ACY1
• NCBI Official Synonym Symbols: ACY-1; ACY1D
• NCBI Protein Information: aminoacylase-1; OTTHUMP00000212459; OTTHUMP00000212462; OTTHUMP00000212463; OTTHUMP00000212464; OTTHUMP00000220363; N-acyl-L-amino-acid amidohydrolase
• UniProt Protein Name: Aminoacylase-1
• Protein Family: Aminoacylase
• UniProt Gene Name: ACY1
• UniProt Entry Name: ACY1_HUMAN

NCBI Description

This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq]

Uniprot Description

ACY1: Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D). ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids. Belongs to the peptidase M20A family.

Protein type: Hydrolase; Amino Acid Metabolism - arginine and proline; EC 3.5.1.14

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cytosol

Molecular Function: metallopeptidase activity; metal ion binding; aminoacylase activity

Biological Process: amino acid metabolic process; xenobiotic metabolic process; proteolysis

Disease: Aminoacylase 1 Deficiency