Mouse alpha Fodrin Monoclonal Antibody | anti-SPTAN1 antibody

alpha Fodrin monoclonal antibody (AA6)

Cat. Num. AAA566414

• Gene Names: SPTAN1; SPECA
• Reactivity: Mammalian, Chicken
• Applications: Immunohistochemistry, Western Blot
• Purity: Partially purified
• Synonyms: alpha Fodrin; Monoclonal Antibody; alpha Fodrin monoclonal antibody (AA6); anti-SPTAN1 antibody

• Host: Mouse
• Reactivity: Mammalian, Chicken
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: AA6
• Purity/Purification: Partially purified
• Form/Format: Liquid. In PBS containing 1.0% BSA and 0.09% sodium azide

• Applicable Applications for anti-SPTAN1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Immunogen: Chicken blood cell membranes following hypotonic lysis and mechanical enucleation
• Preparation and Storage: For long term storage, store at -20 degree C; Store unopened vial at -20 degree C until required for use. AVOID REPEATED FREEZE-THAW CYCLES. Aliquot undiluted antibody into smaller volumes (not less than 10uL) prior to freezing if appropriate. The use of high quality 'antiserum-grade' plastic or glass vials is recommended. Store diluted antibody at 2-4 degree C (do not freeze) and use within 1 month. Dilute to working strength with 50mM Tris-HCl buffer (pH7.6) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-mouse IgG linker antibody is to be used); Shipping: Shipped on Blue Ice

Related Product Information for anti-SPTAN1 antibody

Fodrin, also referred to as non-erythroid (alphaII-; brain) spectrin, is a tetrameric (alphagamma)2 actin-binding, fibrous protein, widely distributed in vertebrates, which forms part of the sub-membranous cytoskeleton within many cell types including neurons, and is particularly abundant with axons. The alpha-subunits of fodrins and spectrin are highly conserved phylogenetically, with the exception of human alpha-fodrin, which shares only 55-59% homology with erythroid-specific alpha-spectrins. The beta-subunits of spectrin (and gamma-subunits of fodrins) are species specific. The interleukin-1 converting enzyme (ICE) family of proteases has been implicated as important effectors of the apoptotic pathway, perhaps acting hierarchically in a protease cascade. Neuronal fodrin is known to be cleaved by calpain following ischaemic insult and it has been proposed that calpain and an unidentified protease play a role in the onset of neuronal death following transient forebrain ischaemia. Recently, an ICE-like protease has been implicated in the early cleavage of fodrin, producing a 150kDa fragment, proximal to CPP32 in Fas-induced and C₂-ceramide mediated apoptosis. A cleavage product of alpha-fodrin has been proposed as a candidate autoantigen in primary Sjögren's syndrome and alpha-fodrin has been shown to be the source of a so-called 'inhibitory protein factor' family, members of which have been shown to inhibit both GABA and ATP-dependent glutamate uptake into purified synaptic vesicles.

Product Categories/Family for anti-SPTAN1 antibody

Cellular Analysis; Cell Organelles & Structures

NCBI and Uniprot Product Information

• NCBI GI #: 110227609
• NCBI GeneID: 374234
• NCBI Accession #: NP_001036003.1
• NCBI GenBank Nucleotide #: NM_001042538.1
• UniProt Accession #: P07751
• Molecular Weight: 285,363 Da
• NCBI Official Full Name: spectrin alpha chain, non-erythrocytic 1
• NCBI Official Synonym Full Names: spectrin, alpha, non-erythrocytic 1
• NCBI Official Symbol: SPTAN1
• NCBI Official Synonym Symbols: SPECA
• NCBI Protein Information: spectrin alpha chain, non-erythrocytic 1; alpha-II spectrin; alpha-spectrin; fodrin alpha chain; nonerythroid alpha-spectrin; spectrin alpha chain, brain; spectrin, alpha, non-erythrocytic 1 (alpha-fodrin); spectrin, non-erythroid alpha chain
• UniProt Protein Name: Spectrin alpha chain, non-erythrocytic 1
• Protein Family: Spectrin
• UniProt Gene Name: SPTAN1
• UniProt Synonym Gene Names: SPTA2
• UniProt Entry Name: SPTN1_CHICK

Uniprot Description

Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.