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Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Mouse anti-Rat Alpha-1-B-Glycoprotein (a1BG) Monoclonal Antibody | anti-a1BG antibody
Monoclonal Antibody to Alpha-1-B-Glycoprotein (a1BG)
Gene Names
A1bg; C44
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Alpha-1-B-Glycoprotein (a1BG); Monoclonal Antibody; Monoclonal Antibody to Alpha-1-B-Glycoprotein (a1BG); anti-a1BG antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
A8
Specificity
The antibody is a mouse monoclonal antibody raised against a1BG. It has been selected for its ability to recognize a1BG in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.39mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- WLDSGSEPE LRAEPQSLLE PWANLTLVCA VDLPTKVFEL IMNGWFLSQV RLETPVLSYR FSLGAITSNN SGVYRCRCGV EPPVDIQLPA LSKWTMLSNA LEVTGKEPLP PPSAHADPVS WITPGGLPVY IMCRVAMRGV TYLLRKEGVD GTQKPDVQHK GTAGFLIYKP GNYSCSYLTH AGGKPSEPSA IVTIKMSAT
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- WLDSGSEPE LRAEPQSLLE PWANLTLVCA VDLPTKVFEL IMNGWFLSQV RLETPVLSYR FSLGAITSNN SGVYRCRCGV EPPVDIQLPA LSKWTMLSNA LEVTGKEPLP PPSAHADPVS WITPGGLPVY IMCRVAMRGV TYLLRKEGVD GTQKPDVQHK GTAGFLIYKP GNYSCSYLTH AGGKPSEPSA IVTIKMSAT
Sequence Length
495
Applicable Applications for anti-a1BG antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant a1BG (Trp22~Thr219) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074687
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P Samples:Rat Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P Samples:Rat Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples:Rat Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples:Rat Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P Samples:Rat Spleen Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P Samples:Rat Spleen Tissue.)
Immunohistochemistry (IHC)
(DABstainingonIHC-PSamples:RatTestisTissue.)
Immunohistochemistry (IHC)
(DABstainingonIHC-PSamples:RatTestisTissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,497 Da
NCBI Official Full Name
alpha-1B-glycoprotein
NCBI Official Synonym Full Names
alpha-1-B glycoprotein
NCBI Official Symbol
A1bg
NCBI Official Synonym Symbols
C44
NCBI Protein Information
alpha-1B-glycoprotein
UniProt Protein Name
Alpha-1B-glycoprotein
Protein Family
UniProt Gene Name
A1bg
NCBI Description
may have a role in the immediate-early response phase of liver regeneration [RGD, Feb 2006]
Uniprot Description
Enzyme and pathway databasesReactome - a knowledgebase of biological pathways and processesMore...ReactomeiR-RNO-114608. Platelet degranulation. R-RNO-6798695. Neutrophil degranulation. Protein family/group databasesMEROPS protease databaseMore...MEROPSiI43.950.
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Product Notes
The a1BG a1bg (Catalog #AAA2012526) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Alpha-1-B-Glycoprotein (a1BG) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-1-B-Glycoprotein (a1BG) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the a1BG a1bg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- WLDSGSEPE LRAEPQSLLE PWANLTLVCA VDLPTKVFEL IMNGWFLSQV RLETPVLSYR FSLGAITSNN SGVYRCRCGV EPPVDIQLPA LSKWTMLSNA LEVTGKEPLP PPSAHADPVS WITPGGLPVY IMCRVAMRGV TYLLRKEGVD GTQKPDVQHK GTAGFLIYKP GNYSCSYLTH AGGKPSEPSA IVTIKMSAT. It is sometimes possible for the material contained within the vial of "Alpha-1-B-Glycoprotein (a1BG), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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