Rabbit anti-Human ALDH4A1 Monoclonal Antibody | anti-ALDH4A1 antibody

Recombinant Anti-ALDH4A1 Antibody, Rabbit Monoclonal

Cat. Num. AAA8106610

• Gene Names: ALDH4A1; P5CD; ALDH4; P5CDh
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A
• Synonyms: ALDH4A1; Monoclonal Antibody; Recombinant Anti-ALDH4A1 Antibody; Rabbit Monoclonal; ALDH4A1 Antibody; Rabbit MAb; aldehyde dehydrogenase 4 family; member A1; Anti-ALDH4 Antibody; Anti-P5CD Antibody; Anti-P5CDh Antibody; anti-ALDH4A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 002
• Specificity: Human ALDH4A1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ALDH4A1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human ALDH4A1 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH4A1 (rh ALDH4A1).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ALDH4A1 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: K562 Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:62 kDaObserved band size:62 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(ALDH4A1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-ALDH4A1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ALDH4A1 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 62 kDaObserved band size: 62 kDa)

Related Product Information for anti-ALDH4A1 antibody

ALDH4A1 is a member of the aldehyde dehydrogenase family. Aldehyde dehydrogenase enzymes function in the metabolism of many molecules including certain fats (cholesterol and other fatty acids) and protein building blocks (amino acids). Additional aldehyde dehydrogenase enzymes detoxify external substances, such as alcohol and pollutants, and internal substances, such as toxins that are formed within cells. ALDH4A1 is expressed abundantly in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas. It is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.

References

Goodman SI, et al. (1974) Defective hydroxyproline metabolism in type II hyperprolinemia. Biochemical medicine. 10 (4): 329-36.Maruyama K, et al. (1994) Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene. 138 (1-2): 171-4.Vasiliou V, et al. (2005) Analysis and update of the human aldehyde dehydrogenase (ALDH) gene family. Hum Genomics. 2 (2): 138-43.

NCBI and Uniprot Product Information

• NCBI GI #: 14043187
• NCBI GeneID: 8659
• Molecular Weight: 56,043 Da
• NCBI Official Full Name: Aldehyde dehydrogenase 4 family, member A1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 4 family member A1
• NCBI Official Symbol: ALDH4A1
• NCBI Official Synonym Symbols: P5CD; ALDH4; P5CDh
• NCBI Protein Information: delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial

NCBI Description

This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]