Mouse anti-Human AKTIP Monoclonal Antibody | anti-AKTIP antibody

AKTIP (FTS, AKT-interacting Protein, Ft1, Fused Toes Protein Homolog, FLJ13258)

Cat. Num. AAA6013586

• Gene Names: AKTIP; FT1; FTS
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: AKTIP; Monoclonal Antibody; AKTIP (FTS; AKT-interacting Protein; Ft1; Fused Toes Protein Homolog; FLJ13258); Anti -AKTIP (FTS; anti-AKTIP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2A11
• Specificity: Recognizes human FTS.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT

• Applicable Applications for anti-AKTIP antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Full length recombinant corresponding to aa1-293 from human FTS (AAH01134) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (58.23kD).)

Western Blot (WB)

(Western Blot analysis of FTS expression in transfected 293T cell line by FTS monoclonal antibody.|Lane 1: FTS transfected lysate (33.1kD).|Lane 2: Non-transfected lysate.)

Related Product Information for anti-AKTIP antibody

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade.

Product Categories/Family for anti-AKTIP antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 61743933
• NCBI GeneID: 64400
• NCBI Accession #: NP_001012398.1
• NCBI GenBank Nucleotide #: NM_001012398.1
• UniProt Accession #: Q9H8T0; Q503B1; Q53H38
• Molecular Weight: 33,128 Da
• NCBI Official Full Name: AKT-interacting protein
• NCBI Official Synonym Full Names: AKT interacting protein
• NCBI Official Symbol: AKTIP
• NCBI Official Synonym Symbols: FT1; FTS
• NCBI Protein Information: AKT-interacting protein; fused toes homolog; fused toes protein homolog
• UniProt Protein Name: AKT-interacting protein
• Protein Family: AKT-interacting protein
• UniProt Gene Name: AKTIP
• UniProt Synonym Gene Names: FTS
• UniProt Entry Name: AKTIP_HUMAN

NCBI Description

The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

AKTIP: an oncogenic membrane-associated protein that is a component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade. Belongs to the ubiquitin-conjugating enzyme family. FTS subfamily. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with HOOK1, HOOK2 and HOOK3. The FHF complex associates with the homotypic vesicular sorting complex (the HOPS complex). Apparently interacts with AKT1. A member of the ubiquitin-conjugating enzyme family, FTS subfamily. Lacks the conserved Cys residue necessary for ubiquitin-conjugating enzyme E2 activity. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, peripheral; Apoptosis; Vesicle

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: Golgi apparatus; cytoplasm; plasma membrane; nucleolus

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding

Biological Process: protein transport; positive regulation of protein binding; endosome organization and biogenesis; lysosome organization and biogenesis; apoptosis; early endosome to late endosome transport; endosome to lysosome transport; protein ubiquitination; positive regulation of protein amino acid phosphorylation