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Immunohistochemistry (IHC) (Anti-AK3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Mouse anti-Human AK3 / Adenylate Kinase 3 Monoclonal Antibody | anti-AK3 antibody

Mouse Monoclonal [clone SJB3-36] (IgG1,k) to Human AK3 / Adenylate Kinase 3

Gene Names
AK3; AK6; FIX; AK3L1; AKL3L; AKL3L1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Protein G Purified
Synonyms
AK3 / Adenylate Kinase 3; Monoclonal Antibody; Mouse Monoclonal [clone SJB3-36] (IgG1; k) to Human AK3 / Adenylate Kinase 3; Anti-AK3 / Adenylate Kinase 3 Antibody (clone SJB3-36) IHC-plus; AK3; AK 3; AKL3L1; Adenylate kinase 3 alpha like; Adenylate kinase 3 like 1; Adenylate kinase 3; Adenylate kinase 6; AK3L1; AK6; AKL3L; FIX; Human AK3; Adenylate Kinase 3; anti-AK3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
SJB3-36
Purity/Purification
Protein G Purified
Form/Format
PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Concentration
1 mg/ml (varies by lot)
Sequence Length
227
Applicable Applications for anti-AK3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IHC-P (20 ug/ml), WB (1:500 - 1:2000)
Usage: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysi...
Target Species
Human
Immunogen Description
Recombinant human AK3 (adenylate kinase isozyme 3) purified from E Coli.
Immunogen Type
Recombinant protein
Immunogen
AK3 / Adenylate Kinase 3 antibody was raised against recombinant human AK3 (adenylate kinase isozyme 3) purified from E Coli.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-AK3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Immunohistochemistry (IHC) (Anti-AK3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Western Blot (WB)

(The recombinant human AK isozymes (AK1(1), AK2(2) and AK3(3)) and mouse liver(4) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. 1: AK1; 2: AK2; 3: AK3; 4: Mouse liver.)

Western Blot (WB) (The recombinant human AK isozymes (AK1(1), AK2(2) and AK3(3)) and mouse liver(4) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. 1: AK1; 2: AK2; 3: AK3; 4: Mouse liver.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,014 Da
NCBI Official Full Name
GTP:AMP phosphotransferase AK3, mitochondrial isoform a
NCBI Official Synonym Full Names
adenylate kinase 3
NCBI Official Symbol
AK3
NCBI Official Synonym Symbols
AK6; FIX; AK3L1; AKL3L; AKL3L1
NCBI Protein Information
GTP:AMP phosphotransferase AK3, mitochondrial; adenylate kinase 3 alpha-like 1; GTP:AMP phosphotransferase, mitochondrial; adenylate kinase 6, adenylate kinase 3 like 1
UniProt Protein Name
GTP:AMP phosphotransferase AK3, mitochondrial
UniProt Gene Name
AK3
UniProt Entry Name
KAD3_HUMAN

NCBI Description

The protein encoded by this gene is a GTP:ATP phosphotransferase that is found in the mitochondrial matrix. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

AK3: is a GTP:ATP phosphotransferase that is found in the mitochondrial matrix. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Protein type: Transferase; EC 2.7.4.10; Nucleotide Metabolism - pyrimidine; Mitochondrial

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: GTP binding; adenylate kinase activity; nucleoside triphosphate adenylate kinase activity; ATP binding

Biological Process: UTP metabolic process; ITP metabolic process; ADP biosynthetic process; blood coagulation; AMP metabolic process; phosphorylation; GTP metabolic process

Research Articles on AK3

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Product Notes

The AK3 ak3 (Catalog #AAA245957) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone SJB3-36] (IgG1,k) to Human AK3 / Adenylate Kinase 3 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AK3 / Adenylate Kinase 3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (20 ug/ml), WB (1:500 - 1:2000) Usage: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysi.. Researchers should empirically determine the suitability of the AK3 ak3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AK3 / Adenylate Kinase 3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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