Mouse anti-Human AHA1 Monoclonal Antibody | anti-AHA1 antibody

AHA1 antibody

Cat. Num. AAA200334

• Gene Names: AHSA1; p38; AHA1; hAha1; C14orf3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: By protein-A affinity chromatography
• Synonyms: AHA1; Monoclonal Antibody; AHA1 antibody; Activator of Hsp90 ATPase-1; Activator of Hsp90 ATPase-1 (AHA1; 19-337); Activator of Hsp90 ATPase protein 1; Hsp90 co-chaperone AHA1; Activator of Hsp90 ATPase-1 Activator of 90 kDa heat shock protein ATPase homolog 1; Activator of heat shock 90kDa protein ATPase homolog 1; AHA 1; AHSA 1; AHSA1; C14orf3; HSPC322; p38.; anti-AHA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, k
• Clone Number: AT3E9
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 338

• Applicable Applications for anti-AHA1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000.
• Antigen Species: Human
• Immunogen: Recombinant human AHA1 (19-337aa) purified from E. coli
• Clone Note: Anti-human AHA1 mAb, clone AT3E9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AHA1 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The Recombinant Human AHA1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AHA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : AHA1 Recombinant protein Lane 2. : MCF-7 cell lysate Lane 3. : PC-3 cell lysate Lane 4. : 293T cell lysate Lane 5. : HeLa cell lysate)

Immunofluorescence (IF)

(ICC/IF analysis of AHA1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AHA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of AHA1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AHA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Related Product Information for anti-AHA1 antibody

The Co-chaperone AHA1 (Activator of HSP90 ATPase-1) is proposed to function as an ATPase activating protein. May affect a step in the endoplasmic reticulum to Golgi trafficking. It expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.

Product Categories/Family for anti-AHA1 antibody

Heat Shock Proteins; Signal Transduction

References

Meyer P., et al. (2004) EMBO. 23 (6): 1402-1410. ; Siligardi G., et al. (2004) J Biol Chem. 270 (50): 51989-51998.

NCBI and Uniprot Product Information

• NCBI GI #: 6912280
• NCBI GeneID: 10598
• NCBI Accession #: NP_036243
• NCBI GenBank Nucleotide #: NM_012111.2
• UniProt Accession #: O95433; Q96IL6; Q9P060; B2R9L2; B4DUR9
• Molecular Weight: 32,340 Da
• NCBI Official Full Name: activator of 90 kDa heat shock protein ATPase homolog 1 isoform 1
• NCBI Official Synonym Full Names: activator of HSP90 ATPase activity 1
• NCBI Official Symbol: AHSA1
• NCBI Official Synonym Symbols: p38; AHA1; hAha1; C14orf3
• NCBI Protein Information: activator of 90 kDa heat shock protein ATPase homolog 1
• UniProt Protein Name: Activator of 90 kDa heat shock protein ATPase homolog 1
• Protein Family: ATPase
• UniProt Gene Name: AHSA1
• UniProt Synonym Gene Names: C14orf3; AHA1

Uniprot Description

Acts as a co-chaperone of HSP90AA1. Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity. Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360).