Mouse anti-Human AGO1 Monoclonal Antibody | anti-AGO1 antibody

AGO1 (Protein Argonaute-1, hAgo1, Argonaute1, Eukaryotic Translation Initiation Factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding Protein Q99, EIF2C1) (FITC)

Cat. Num. AAA6243577

• Gene Names: AGO1; Q99; EIF2C; hAgo1; EIF2C1; GERP95
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: AGO1; Monoclonal Antibody; AGO1 (Protein Argonaute-1; hAgo1; Argonaute1; Eukaryotic Translation Initiation Factor 2C 1; eIF-2C 1; eIF2C 1; Putative RNA-binding Protein Q99; EIF2C1) (FITC); anti-AGO1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 13A152
• Specificity: Recognizes human AGO1at ~97kD. Does not react with Ago2, Ago3 or Ago4.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-AGO1 antibody: Western Blot (WB)
• Immunogen: Recombinant protein corresponding to human AGO1 fused to GST-tag.
• Conjugate: FITC
• Preparation and Storage: Store at -20 degree C.

Testing Data

(Representative lot data. HeLa cell lysate was probed with MBS646135 (0.1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse IgG secondary antibody and a chemiluminescence detection system. Arrow indicates Ago 1 (~97kD).)

Product Categories/Family for anti-AGO1 antibody

Antibodies; Translation Initiation Factors

References

1. Azuma-Mukai A., et al. (2008) Proc. Natl. Acad. Sci. USA. 105: 7964-7969. 2. Kim, D.H., et al. (2008). Proc Natl Acad Sci USA. 105(42):16230-16235. 3. Sasaki, T., et al. (2003). Genomics. 82(3):323- 330. 3. Janowski, B.A., et al. (2006). Nat Struct Mol Biol. 13(9):787-792. 4. Ma, J.B., Ye, K., and Patel, D.J. (2004). Nature. 429(6989):318-322.

NCBI and Uniprot Product Information

• NCBI GI #: 6912352
• NCBI GeneID: 26523
• NCBI Accession #: NP_036331
• NCBI GenBank Nucleotide #: NM_012199
• UniProt Accession #: Q9UL18
• Molecular Weight: 97kDa
• NCBI Official Full Name: protein argonaute-1 isoform 1
• NCBI Official Synonym Full Names: argonaute RISC component 1
• NCBI Official Symbol: AGO1
• NCBI Official Synonym Symbols: Q99; EIF2C; hAgo1; EIF2C1; GERP95
• NCBI Protein Information: protein argonaute-1
• UniProt Protein Name: Protein argonaute-1
• Protein Family: Protein argonaute
• UniProt Gene Name: AGO1
• UniProt Synonym Gene Names: EIF2C1; Argonaute1; hAgo1; eIF-2C 1; eIF2C 1
• UniProt Entry Name: AGO1_HUMAN

NCBI Description

This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]

Uniprot Description

AGO1: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, EIF2C2/AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with LIMD1, WTIP and AJUBA. Belongs to the argonaute family. Ago subfamily.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; polysome; cytoplasm; cytosol; ribonucleoprotein complex

Molecular Function: endoribonuclease activity; protein binding; miRNA binding; RNA binding

Biological Process: epidermal growth factor receptor signaling pathway; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; innate immune response; gene expression; positive regulation of transcription from RNA polymerase II promoter; posttranscriptional gene silencing