Western Blot (WB)
(Western Blot Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate All lanes: ADORA1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 14 kDa Observed band size: 37 kDa)
ADORA1 recombinant antibody
ADORA1 Antibody
IHC: 1:50-1:200
Western Blot (WB)
(Western Blot Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate All lanes: ADORA1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 14 kDa Observed band size: 37 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate All lanes: ADORA1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 14 kDa Observed band size: 37 kDa)
Immunohistochemistry (IHC)
(IHC image of MBS7136558 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image of MBS7136558 diluted at
1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ADORA1: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Apoptosis; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: asymmetric synapse; presynaptic membrane; postsynaptic membrane; cell soma; endoplasmic reticulum; basolateral plasma membrane; integral to plasma membrane; postsynaptic density; presynaptic active zone; plasma membrane; dendritic spine; terminal button; axolemma
Molecular Function: protein binding; G-protein-coupled receptor binding; protein heterodimerization activity; G-protein beta/gamma-subunit binding; purine nucleoside binding; adenosine receptor activity, G-protein coupled; phospholipase C activity; heterotrimeric G-protein binding
Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; relaxation of vascular smooth muscle; activation of MAPKK activity; negative regulation of glutamate secretion; negative regulation of hormone secretion; positive regulation of potassium ion transport; regulation of synaptic plasticity; positive regulation of peptide secretion; neurological control of breathing; thermoregulation; signal transduction; positive regulation of protein amino acid dephosphorylation; negative regulation of cell proliferation; regulation of glomerular filtration; cell-cell signaling; positive regulation of nucleoside transport; positive regulation of epidermal growth factor receptor activity; negative regulation of lipid catabolic process; negative regulation of vasodilation; negative regulation of blood pressure; detection of temperature stimulus involved in sensory perception of pain; protein targeting to membrane; inflammatory response; negative regulation of synaptic transmission, GABAergic; adenosine receptor signaling pathway; nervous system development; negative regulation of neurotrophin production; negative regulation of synaptic transmission, glutamatergic; negative regulation of acute inflammatory response; negative regulation of leukocyte migration; phagocytosis; response to hypoxia; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; cognition; positive regulation of blood pressure; lipid catabolic process; negative regulation of apoptosis
Research Articles on ADORA1
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Product Notes
The ADORA1 adora1 (Catalog #AAA7136558) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADORA1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADORA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) . WB: 1:500-1:5000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ADORA1 adora1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADORA1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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