Mouse Adenovirus Fiber Monoclonal Antibody
Adenovirus Fiber (Monomer and Trimer)
Applications
Western Blot, Immunofluorescence
Purity
Purified by Protein A affinity chromatography from ascites.
Synonyms
Adenovirus Fiber; Monoclonal Antibody; Adenovirus Fiber (Monomer and Trimer); Anti -Adenovirus Fiber (Monomer and Trimer); anti-Adenovirus Fiber antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3F13
Specificity
Recognizes both monomers and trimers of Adenovirus, as judged by reactivity with both boiled (monomers (62kD) only) and unboiled (both monomers and trimers (180-200kD bands)) fiber on Western Blots and by indirect Immunofluorescence studies of cells infected with a temperature-sensitive Ad5 fiber mutant (H5ts142) which does not form trimers at the nonpermissive temperature at both 32 degree C and 39.5 degree C.
Purity/Purification
Purified by Protein A affinity chromatography from ascites.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. Please inquire.
Concentration
0.2mg/ml (varies by lot)
Applicable Applications for anti-Adenovirus Fiber antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Not suitable for the Inhibition of Ad2 attachment. Other applications not tested. Optimal dilutions to be determined by the researcher.
Immunogen
UV-irradiated Ad2 virus. Epitope: N-terminus, probably the first 17aa. Cellular Localization: Nuclear.
Grade
Affinity Purified
Positive Control
Adenovirus infected cells and tissues
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Adenovirus Fiber antibody
Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the type-specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the virion.
Product Categories/Family for anti-Adenovirus Fiber antibody
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Product Notes
The Adenovirus Fiber (Catalog #AAA604315) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Adenovirus Fiber can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Not suitable for the Inhibition of Ad2 attachment. Other applications not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Adenovirus Fiber for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adenovirus Fiber, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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