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Western Blot (WB) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3. : TF-1 cell lysate Lane 4. : NIH/3T3 cell lysate Lane 5. : HepG2 cell lysate)

Mouse anti-Human ACP1 Monoclonal Antibody | anti-ACP1 antibody

ACP1 antibody

Gene Names
ACP1; HAAP; LMWPTP; LMW-PTP
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
ACP1; Monoclonal Antibody; ACP1 antibody; Acid phosphatase 1 soluble isoform b; Acid phosphatase 1; soluble isoform b; HAAP; LMW-PTP; Red cell acid phosphatase 1; Adipocyte acid phosphatase; soluble isoform b Acid phosphatase 1 soluble; Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase; Low molecular weight phosphotyrosine protein phosphatase; Acid phosphatase of erythrocyte; PAP2; Protein tyrosine phosphatase; PTPase; Purple acid phosphatase; Red cell acid phosphatase 1.; anti-ACP1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT5C11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
158
Applicable Applications for anti-ACP1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human ACP1 (1-158aa) purified from E. coli
Clone Note
Anti-human ACP1 mAb, clone AT5C11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACP1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3. : TF-1 cell lysate Lane 4. : NIH/3T3 cell lysate Lane 5. : HepG2 cell lysate)

Western Blot (WB) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3. : TF-1 cell lysate Lane 4. : NIH/3T3 cell lysate Lane 5. : HepG2 cell lysate)

Immunofluorescence (IF)

(ICC/IF analysis of ACP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Immunofluorescence (IF) (ICC/IF analysis of ACP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-ACP1 antibody
Low molecular weight phosphotyrosine protein phosphatase, also known as ACP1, is an enzyme that catalyzes the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols such as methanol and glycerol. And, It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate.
Product Categories/Family for anti-ACP1 antibody
References
Junien C., et al. (1979) Hum Genet. 48 (1): 17-21.; Dissing J., et al. (1992) Biochim Biophys Acta. 1121: 261-268.; Lazaruk KD., et al. (1993) Biochem Biophys Res Commun. 196: 440-446.; Bryson GL., et al. (1995) Genomics. 30: 133-140.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
52
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,230 Da
NCBI Official Full Name
low molecular weight phosphotyrosine protein phosphatase isoform b
NCBI Official Synonym Full Names
acid phosphatase 1
NCBI Official Symbol
ACP1
NCBI Official Synonym Symbols
HAAP; LMWPTP; LMW-PTP
NCBI Protein Information
low molecular weight phosphotyrosine protein phosphatase
UniProt Protein Name
Low molecular weight phosphotyrosine protein phosphatase
Protein Family
UniProt Gene Name
ACP1
UniProt Synonym Gene Names
LMW-PTP; LMW-PTPase

NCBI Description

The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.

Research Articles on ACP1

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Product Notes

The ACP1 acp1 (Catalog #AAA200379) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ACP1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the ACP1 acp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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