Mouse anti-Human ABCF1 Monoclonal Antibody | anti-ABCF1 antibody

Mouse monoclonal antibody Anti-Human ABCF1

Cat. Num. AAA120004

• Gene Names: ABCF1; ABC27; ABC50
• Reactivity: Human
• Applications: Dot Blot, Immunoprecipitation
• Synonyms: ABCF1; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human ABCF1; ATP-binding cassette; ABC27; anti-ABCF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: ABC5H06
• Form/Format: Mouse monoclonal anti-human ABCF1 antibody in PBS (3.0 mM KCl, 1.5 mM KH PO , 140 mM NaCl, 8.0 mM Na HPO (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN ).
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 3204

• Applicable Applications for anti-ABCF1 antibody: Dot Blot (DB), Immunoprecipitation (IP)
• Application Notes: WB: 0.2-2 ug/ml; IP: 100-500 ug/sample
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Preparation and Storage: Store at 2-8 C for up to one year. We recommend storing at –20 C for long-term storage. Avoid repeat freezing and thawing cycles.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-ABCF1 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Immunoprecipitation (IP)

(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-ABCF1 mAb. [Lot No. ABC5H06-1]Predicted molecular weight: 95 kDa)

Related Product Information for anti-ABCF1 antibody

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 34783469
• NCBI GeneID: 23
• NCBI Official Full Name: Homo sapiens ATP-binding cassette, sub-family F (GCN20), member 1, mRNA
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family F (GCN20), member 1
• NCBI Official Symbol: ABCF1
• NCBI Official Synonym Symbols: ABC27; ABC50
• NCBI Protein Information: ATP-binding cassette sub-family F member 1; ATP-binding cassette 50 (TNF-alpha stimulated); TNF-alpha-stimulated ABC protein; TNFalpha-inducible ATP-binding protein
• UniProt Protein Name: ATP-binding cassette sub-family F member 1
• Protein Family: ABC transporter F family
• UniProt Gene Name: ABCF1
• UniProt Entry Name: ABCF1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCF1: Isoform 2 is required for efficient Cap- and IRES- mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis. Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: nucleoplasm; polysomal ribosome; membrane; cytoplasm; ribosome; nuclear envelope

Molecular Function: protein binding; translation activator activity; translation factor activity, nucleic acid binding; ATPase activity; ribosome binding; ATP binding

Biological Process: positive regulation of translation; translation; ribosome biogenesis and assembly; translational initiation; inflammatory response